Human beta-Catenin Antibody (196618)

R&D Systems | Catalog # MAB13291

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 196618
View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human beta -Catenin
aa 2-781

Specificity

Detects human  beta -Catenin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human beta-Catenin Antibody (196618)

beta -Catenin antibody in SW480 Human Cell Line by Immunocytochemistry (ICC).

beta ‑Catenin in SW480 Human Cell Line.

beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human beta -Catenin Monoclonal Antibody (Catalog # MAB13291) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta -Catenin antibody in Human Pancreas by Immunohistochemistry (IHC-P).

beta ‑Catenin in Human Pancreas.

beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human beta -Catenin Monoclonal Antibody (Catalog # MAB13291) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human beta-Catenin by Immunocytochemistry/Immunofluorescence

Detection of Human beta-Catenin by Immunocytochemistry/Immunofluorescence

Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human beta-Catenin Antibody (196618)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human neuroectodermals differentiated from embryonic stem cells and immersion fixed SW480 human colorectal adenocarcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Western Blot

1 µg/mL
Sample: Recombinant Human beta -Catenin

Reviewed Applications

Read 5 reviews rated 4.8 using MAB13291 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-Catenin

Beta-Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as as transcriptional co-activator in the nucleus and is also involved the canonical Wnt signal transduction pathways.

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human); 12387 (Mouse); 84353 (Rat)

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for Human beta-Catenin Antibody (196618)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human beta-Catenin Antibody (196618)

For research use only

Citations for Human beta-Catenin Antibody (196618)

Customer Reviews for Human beta-Catenin Antibody (196618) (5)

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Human beta-Catenin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Bone marrow cells
    Species: Human
    Verified Customer | Posted 11/02/2021
    Human beta-Catenin Antibody (196618) MAB13291
  • Human beta -Catenin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 11/03/2017
    Human beta-Catenin Antibody (196618) MAB13291
  • Human beta-Catenin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: SpecificSensitivity: SensitiveBuffer: 5% milk in TBSTDilution: 1/500
    Human beta-Catenin Antibody (196618) MAB13291
  • Human beta-Catenin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: SpecificSensitivity: SensitiveBuffer: 1% BSA + 0.3% Triton X-100 in PBSDilution: 1/100
    Human beta-Catenin Antibody (196618) MAB13291
  • Human beta-Catenin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Reasonably specificSensitivity: Reasonably sensitiveBuffer: 1% BSA + 0.3% Triton X-100 in PBSDilution: 1/62.5
    Human beta-Catenin Antibody (196618) MAB13291

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