Human BMP-2 Antibody (100221)

Human BMP-2 ELISA Standard Curve

Recombinant Human/Mouse/Rat BMP‑2 (Catalog # 355-BM) was serially diluted and captured by Mouse Anti-Human BMP‑2 Monoclonal Antibody (Catalog # MAB3551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BMP‑2/BMP‑4 Monoclonal Antibody (Catalog # MAB3552) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Detection of BMP-2 by Western Blot

Reduction of osteogenic markers under pharmacological inhibition of TRPM4.A and F, Representative western blots of BMP2, Runx2, alpha SMA, and TRPM4 protein expression in hVICs after 7 (A) and 14 days (F) of culture. As indicated under panels, cells were cultured in SM conditions or in PM conditions without or with 9 PHE 3.10−6 mol.L−1. B–E and G–J, Quantification of intensity labeling of BMP2 (N=9 and 7 for B and G); Runx2 (N=9 and 14 for C and H); alpha SMA (N=10 and 8 for D and I) and TRPM4 (N=8 and 12 for E and J) after 7 days (B–E) and 14 days (G–J) of hVIC maintained in SM, PM, and PM+9 PHE culture conditions. Histograms are mean±SEM of fold change vs PM. Analyzed with Friedman (B, C, G, H, J) with Dunn's multiple comparisons and 1‐way ANOVA (D, E, I) with uncorrected Fisher's least significant difference multiple comparisons test. *P<0.05, **P<0.01, ***P<0.001, ns=nonsignificant. 9 PHE indicates 9‐phenanthrol; BMP2, bone morphogenetic protein 2; hVIC, human valvular interstitial cell; PM, pro‐calcifying media; Runx2, runt‐related transcription factor 2; SM, standard media; alpha SMA, alpha ‐smooth muscle actin; and TRPM4, transient receptor potential melastatin 4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40194969), licensed under a CC-BY license. Not internally tested by R&D Systems.