Human BMPR-IB/ALK-6 Antibody (477914)

R&D Systems | Catalog # MAB5051

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 477914
View all BMPR-IB/ALK-6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMPR‑IB/ALK‑6
Lys14-Arg126
Accession # O00238

Specificity

Detects human BMPR‑IB/ALK‑6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse BMPR-IB is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human BMPR-IB/ALK-6 Antibody (477914)

Detection of BMPR‑IB/ALK‑6 antibody in PC‑3 Human Cell Line antibody by Flow Cytometry.

Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR-IB/ ALK-6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
Detection of BMPR-IB/ALK-6 antibody in Human iPS cells differentiated to Mesoderm antibody by Flow Cytometry.

Detection of BMPR-IB/ALK-6 in Human iPS cells differentiated to Mesoderm by Flow Cytometry.

Human iPS cells differentiated to mesoderm (using Catalog # SC030B) were stained with Mouse Anti-Human BMPR-IB/ALK-6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibosy (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
BMPR-IB/ALK-6 antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC).

BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line.

BMPR-IB/ALK-6 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human BMPR-IB/ALK-6 Monoclonal Antibody (Catalog # MAB5051) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human BMPR-IB/ALK-6 Antibody (477914)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: PC‑3 human prostate cancer cell line and human iPS cells differentiated to mesoderm (using Catalog # SC030B)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line

Reviewed Applications

Read 1 review rated 5 using MAB5051 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% amino acid sequence identity.

References

  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
  2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Long Name

Bone Morphogenetic Protein Receptor IB

Alternate Names

ALK-6, BMPR1B, BMPRIB, CDw293

Entrez Gene IDs

658 (Human); 12167 (Mouse)

Gene Symbol

BMPR1B

UniProt

O00238

Additional BMPR-IB/ALK-6 Products

Product Documents for Human BMPR-IB/ALK-6 Antibody (477914)

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Product Specific Notices for Human BMPR-IB/ALK-6 Antibody (477914)

For research use only

Related Research Areas

BMP Family
Mesenchymal Stem Cell Markers

Citations for Human BMPR-IB/ALK-6 Antibody (477914)

Customer Reviews for Human BMPR-IB/ALK-6 Antibody (477914) (1)

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  • Human BMPR-IB/ALK-6 Antibody
    Name: Alina Filatova
    Application: Flow Cytometry
    Sample Tested: Cells from neural differentiation of hiPSCs
    Species: Human
    Verified Customer | Posted 10/20/2017
    The cells were stained with 1µl of anti-BMPR1 antibody in 100 µl FACS staining buffer for 30 min at 4C. After washing, the cells were incubated with 1µl of anti-mouse IgG2b-APC antibody in 100 µl FACS staining buffer for 20 min at 4C.
    Human BMPR-IB/ALK-6 Antibody (477914) MAB5051

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