Human Caldesmon/CALD1 Antibody (767001)

R&D Systems | Catalog # MAB7569

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 767001
View all Caldesmon/CALD1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Caldesmon/CALD1
Lys696-Val793
Accession # Q05682

Specificity

Detects human Caldesmon/CALD1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Caldesmon/CALD1 Antibody (767001)

Detection of Human Caldesmon/CALD1 antibody by Western Blot.

Detection of Human Caldesmon/CALD1 by Western Blot.

Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caldesmon/CALD1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Caldesmon/CALD1 antibody in WI-38 Human Cell Line by Immunocytochemistry (ICC).

Caldesmon/CALD1 in WI‑38 Human Cell Line.

Caldesmon/CALD1 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Caldesmon/CALD1 in Endometrial Carcinoma.

Caldesmon/CALD1 was detected in immersion fixed paraffin-embedded sections of endometrial carcinoma using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Caldesmon/CALD1 in Endometrial Carcinoma.

Caldesmon/CALD1 was detected in immersion fixed paraffin-embedded sections of melanoma using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Caldesmon/CALD1 Antibody (767001)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed WI‑38 human lung fibroblast cell line

Immunohistochemistry

3-25 µg/mL
Sample:

Immersion fixed paraffin-embedded sections of endometrial carcinoma and melanoma

Western Blot

2 µg/mL
Sample: WI‑38 human lung fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caldesmon/CALD1

Caldesmon, also known as CaD or h-CaD, is a 120-150 kDa cytosolic protein that is important in actin cytoskeleton dynamics. Smooth muscle Caldesmon regulates calcium-dependent smooth muscle contraction by stabilizing actin filaments, inhibiting the actomyosin ATPase, and interacting with F-actin, myosin, tropomyosin, and calmodulin. It is also a key molecule in other related actin-related processes including cytokinesis, cell migration, wound healing, and exocytosis. Caldesmon activity is regulated by serine and tyrosine phosphorylation by multiple kinases. Alternate splicing generates 70-80 kDa isoforms of human Caldesmon, known as I-CaD, that lack the central repeating region of the protein (aa 208-436 or aa 208-462). These isoforms are widely expressed and are comparable in size to full length mouse and rat Caldesmon. Within aa 696-793, human Caldesmon shares 94% and 95% aa sequence identity with mouse and rat Caldesmon, respectively.

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Entrez Gene IDs

800 (Human)

Gene Symbol

CALD1

UniProt

Q05682 (Human)

Additional Caldesmon/CALD1 Products

Product Documents for Human Caldesmon/CALD1 Antibody (767001)

Certificate of Analysis

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Product Specific Notices for Human Caldesmon/CALD1 Antibody (767001)

For research use only

Citations for Human Caldesmon/CALD1 Antibody (767001)

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Protocols

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