Human Calsyntenin-1 Antibody

R&D Systems | Catalog # AF5346

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Calsyntenin-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Calsyntenin-1 isoform 2
Arg30-Thr851
Accession # AAH33902

Specificity

Detects human Calsyntenin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Calsyntenin-2 and rhCalsyntenin-3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Calsyntenin-1 Antibody

Detection of Human Calsyntenin‑1 antibody by Western Blot.

Detection of Human Calsyntenin‑1 by Western Blot.

Western blot shows lysates of human brain cortex and brain cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Calsyntenin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5346) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Calsyntenin-1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Calsyntenin-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain cortex and brain cerebellum tissue

Reviewed Applications

Read 1 review rated 5 using AF5346 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calsyntenin-1

Calsyntenin-1 (CST-1; also Alcadein-alpha ) is a 140‑150 kDa member of the Alcadein family of molecules. It is expressed in the ER/Golgi and on the plasma membranes of almost all neurons. It is a calcium-binding protein that may regulate post-synaptic signaling and APP cleavage and gene activation. Notably, when cleaved in a manner similar to APP, its intracellular fragment antagonizes APP AICD gene activation. Mature human CST-1 is a 953 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 38‑265) in its extracellular region (aa 29‑859) and a 101 aa cytoplasmic tail. Extracellular cleavage occurs at Met825 Ala826, generating a soluble 115 kDa ECD. Isoforms involve a 78 aa substitution for aa 917‑981, plus deletions of aa 72‑81 and 507‑525. Over aa 30‑851, human CST-1 shares 92% aa identity with mouse CST-1.

Alternate Names

Alcadein-alpha, Calsyntenin1, CLSTN1, CS1, CSTN1, XB31alpha

Entrez Gene IDs

22883 (Human); 65945 (Mouse); 313717 (Rat)

Gene Symbol

CLSTN1

UniProt

AAH33902

Additional Calsyntenin-1 Products

Product Documents for Human Calsyntenin-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Calsyntenin-1 Antibody

For research use only

Customer Reviews for Human Calsyntenin-1 Antibody (1)

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  • Human Calsyntenin-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 08/24/2020
    This antibody could recognize both the full-length and the cleaved N-terminus of endognous Clstn1. In the Clstn1 knockdown sample, both bands clearly disappeared.
    Human Calsyntenin-1 Antibody AF5346

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