Human CCL17/TARC Antibody [Biotin]

R&D Systems | Catalog # BAF364

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL17/TARC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL17/TARC (R&D Systems, Catalog # 364-DN)
Ala24-Ser94
Accession # Q92583

Specificity

Detects human CCL17/TARC in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant mouse TARC, recombinant human (rh) MIP-1 alpha, rhMIP-1 beta, rhRANTES, rhMCP‑1, rhTECK, and rh6Ckine is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CCL17/TARC Antibody [Biotin]

Human CCL17 / TARC ELISA Standard Curve

Human CCL17 / TARC ELISA Standard Curve

Recombinant Human CCL17/TARC (Catalog # 364-DN) was serially diluted and captured by Mouse Anti-Human CCL17/TARC Monoclonal Antibody (Catalog # MAB364) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL17/TARC Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL17/TARC (Catalog # 364-DN)

Human CCL17/TARC Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CCL17/TARC Antibody (Catalog # MAB364)
  • Standard: Recombinant Human CCL17/TARC Protein (Catalog # 364-DN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF364 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL17/TARC

CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

References

  1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
  2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
  3. Nomiyama, H. et al. (1997) Genomics 40:211.

Alternate Names

ABCD-2, SCYA17, TARC

Entrez Gene IDs

6361 (Human); 20295 (Mouse)

Gene Symbol

CCL17

UniProt

Q92583

Additional CCL17/TARC Products

Product Documents for Human CCL17/TARC Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL17/TARC Antibody [Biotin]

For research use only

Citations for Human CCL17/TARC Antibody [Biotin]

Customer Reviews for Human CCL17/TARC Antibody [Biotin] (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human CCL17/TARC Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant and Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/22/2017
    The TARC ELISA we made used MAB364 as the capture and BAF364 as the detection with recombinant antige 364-DN as the immunoassay standard.
    Human CCL17/TARC Antibody [Biotin] BAF364
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 21801246
    Species: Human
    Verified Customer | Posted 01/13/2015

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