Human CCL2/JE/MCP-1 Antibody (24822)

R&D Systems | Catalog # MAB279

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, ELISA Capture, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 24822
View all CCL2/JE/MCP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL2/JE/MCP-1

Specificity

Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human (rh) CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL1, 2, 3, 4, 5, 6, 7, 9/10MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or rrCCL20.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL2/JE/MCP-1 Antibody (24822)

Detection of Recombinant Human CCL2/JE/MCP-1 antibody by Western Blot.

Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot.

Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Human CCL8/ MCP-2 (Catalog # 281-CP), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL2/JE/ MCP-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Human CCL2/JE/MCP‑1 Antibody.

Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Human CCL2/JE/MCP‑1 Antibody.

Recombinant Human CCL2/JE/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/ MCP-1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL2/JE/ MCP-1 Monoclonal Antibody (Catalog # MAB279). The ND50 is typically 0.9-4.5 µg/mL.

Applications for Human CCL2/JE/MCP-1 Antibody (24822)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC)

Neutralization

Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.9-4.5 µg/mL in the presence of 0.15 µg/mL Recombinant Human CCL2/JE/MCP‑1.

Reviewed Applications

Read 5 reviews rated 4.6 using MAB279 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

Additional CCL2/JE/MCP-1 Products

Product Documents for Human CCL2/JE/MCP-1 Antibody (24822)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL2/JE/MCP-1 Antibody (24822)

For research use only

Citations for Human CCL2/JE/MCP-1 Antibody (24822)

Customer Reviews for Human CCL2/JE/MCP-1 Antibody (24822) (5)

4.6 out of 5
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  • Human CCL2/JE/MCP-1 Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Primate
    Verified Customer | Posted 11/28/2022
    Used as capture antibody in our NHP ELISA
    Human CCL2/JE/MCP-1 Antibody (24822) MAB279
  • Human CCL2/JE/MCP-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Glioblastoma
    Species: Human
    Verified Customer | Posted 11/07/2021
    Human CCL2/JE/MCP-1 Antibody (24822) MAB279
  • Human CCL2/JE/MCP-1 Antibody
    Name: Zoia Levashova
    Application: MSD assay
    Sample Tested: Vitreous humor
    Species: Cynomolgus Monkey
    Verified Customer | Posted 11/20/2018
    After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC). Paired with SulfoTag-modified AF-279-NA as a detection antibody. Calibration curves with Recombinant Human CCL2 (279-MC-010/CF) is shown (dynamic range 6-25,000 pg/ml)
    Human CCL2/JE/MCP-1 Antibody (24822) MAB279
  • Human CCL2/JE/MCP-1 Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/20/2017
  • Human CCL2/JE/MCP-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/20/2017
    This antibody was used with BAF279 to create a sandwich ELISA to measure MCP-1 in human serum samples.
    Human CCL2/JE/MCP-1 Antibody (24822) MAB279

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