Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 62714
View all CCL28 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL28
Ile23-Tyr127
Accession # Q9NRJ3

Specificity

Detects human CCL28 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL27 or recombinant mouse CCL28 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CCL28 Antibody (62714)

Human CCL28 ELISA Standard Curve

Human CCL28 ELISA Standard Curve

Recombinant Human CCL28 (Catalog # 717-VC) was serially diluted and captured by Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB7171) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF717) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL28 Antibody (62714)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL28 (Catalog # 717-VC)

Human CCL28 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CCL28 Biotinylated Antibody (Catalog # BAF717)
  • Standard: Recombinant Human CCL28 Protein (Catalog # 717-VC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL28

Human CCL28 (CC chemokine ligand 28) is a member of the CC chemokine superfamily. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 share 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Human and mouse CCL28 RNA is most highly expressed by epithelial cells in normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. The receptor for CCL28 is the CCR10 (formerly GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

References

  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Alternate Names

CCL28, MEC, VIC

Entrez Gene IDs

56477 (Human); 56838 (Mouse)

Gene Symbol

CCL28

UniProt

Q9NRJ3

Additional CCL28 Products

Product Documents for Human CCL28 Antibody (62714)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL28 Antibody (62714)

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Human CCL28 Antibody (62714)

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