Human CCR1 Antibody (53504)

R&D Systems | Catalog # MAB145

Clone 53504 was used by HLDA to establish CD designation
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Assay Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 53504
View all CCR1 Primary Antibodies »

Product Specifications

Immunogen

Swiss 3T3 mouse cell line transfected with human CCR1
Accession # P32246

Specificity

Detects human CCR1. Reacts specifically with human CCR1 transfectants and not the parental cell line in flow cytometry.No cross‑reactivity with CCR5 transfectants is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CCR1 Antibody (53504)

Detection of CCR1 in PBMC Monocytes by Flow Cytometry

PBMC Monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB145) or (B) isotype control antibody (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR1 by Immunocytochemistry/ Immunofluorescence

Detection of CCR1 by Immunocytochemistry/ Immunofluorescence

AVPR1A ligands interfere with heteromerization between AVPR1A and CR partners.(A, C) THP-1 cells were incubated with vehicle (ctrl., top), 10 μmol/liter arginine vasopressin (aVP, center), or 10 μmol/liter conivaptan (con, bottom) for 30 min at 37°C, and the cell surface expression of individual receptors (A) and receptor–receptor proximity (C) was visualized by the proximity ligation assay (PLA). Images show merged DAPI (nuclear counterstain) and PLA signals (red, lambda excitation/emission 598/634 nm) acquired from z-stack images (n = 10; thickness 0.5 μm, bottom to top) and are representative of n = 3 independent experiments. Scale bar, 10 μm. (B) Quantification of PLA signals for AVPR1A, CCR1, CCR2, CCR8, CXCR1, and CXCR4 from n = 3 experiments. Data are the mean ± SE. *P < 0.05 versus ctrl. (D) Quantification of PLA signals for receptor–receptor proximity between AVPR1A and CCR1, CCR2, CCR8, or CXCR4 from n = 3 experiments. Data are the mean ± SE. *P < 0.05 versus ctrl. Image collected and cropped by CiteAb from the following open publication (https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.202402657), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCR1 by Immunocytochemistry/ Immunofluorescence

Detection of CCR1 by Immunocytochemistry/ Immunofluorescence

CR:AVPR1A heteromers are detectable in THP-1 cells and human monocytes.(A, B) Representative proximity ligation assay images for the detection of individual receptors ((A, B), top rows) and receptor–receptor proximity ((A, B), bottom rows) in THP-1 cells (A) and human monocytes (B). Images show merged DAPI (nuclear counterstain) and proximity ligation assay signals (red, lambda excitation/emission 598/634 nm) acquired from z-stack images (n = 10; thickness = 0.5 μm, bottom to top) and are representative of n = 3 independent experiments. As controls, cells were incubated with IgG (A/B, top) or a combination of IgG and anti-AVPR1A (not shown). (A, B) Scale bars, (A) 10 μm and (B) 5 μm. Image collected and cropped by CiteAb from the following open publication (https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.202402657), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CCR1 Antibody (53504)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood monocytes

Reviewed Applications

Read 1 review rated 4 using MAB145 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR1

CCR1 is a 7 transmembrane domain chemokine receptor expressed on T cells, B cells, monocytes, dendritic cells, and eosinophils. It binds the chemokines CCL3, 4, 5, and 7.

Alternate Names

CCR1, CD191

Entrez Gene IDs

1230 (Human); 12768 (Mouse); 57301 (Rat)

Gene Symbol

CCR1

UniProt

P32246

Additional CCR1 Products

Product Documents for Human CCR1 Antibody (53504)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCR1 Antibody (53504)

For research use only

Citations for Human CCR1 Antibody (53504)

Customer Reviews for Human CCR1 Antibody (53504) (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: rabbit spleen primary isolated cells
    Species: Other
    Verified Customer | Posted 01/29/2015
    rabbit spleen primary cells IHC with CCR1
    Human CCR1 Antibody (53504) MAB145

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