Human CD-M6PR Lumenal Domain Antibody

R&D Systems | Catalog # AF5320

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD-M6PR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD-M6PR Lumenal Domain
Thr27-His185
Accession # P20645

Specificity

Detects human CD-M6PR Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross‑reactivity with recombinant mouse CD-M6PR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD-M6PR Lumenal Domain Antibody

Detection of Human CD-M6PR Lumenal Domain antibody by Western Blot.

Detection of Human CD-M6PR Lumenal Domain by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD-M6PR Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD-M6PR Lumenal Domain at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human CD-M6PR Lumenal Domain Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD-M6PR

The CD-M6PR (cation-dependent mannose 6-phosphate receptor; also CD-MPR) is a 44‑46 kDa member of the P-type lectin family of proteins. It is ubiquitously expressed, found internally, and transports newly formed mannose 6-phosphate bearing acid hydrolases from the secretory pathway to acidified lysosomes. Mature human CD-M6PR is a type I transmembrane glycoprotein that is 251 amino acids (aa) in length. It functions as a homodimer, and contains a 159 aa luminal region (aa 27‑185) plus a 67 aa cytoplasmic domain. The aa residues Gln66, Arg111, Glu133 and Tyr143 recognize carbohydrate; Asp103 interacts with divalent cation (Mn2+), increasing ligand affinity and receptor oligomerization. Over aa 27‑184, human CD-M6PR shares 90% aa identity with mouse CD-M6PR.

Long Name

Cation Dependent Mannose-6-Phosphate Receptor 46 kDa

Alternate Names

CD-MPR, CDM6PR, M6PR, MPR46, MPRD, SMPR

Entrez Gene IDs

4074 (Human); 17113 (Mouse); 312689 (Rat)

Gene Symbol

M6PR

UniProt

P20645

Additional CD-M6PR Products

Product Documents for Human CD-M6PR Lumenal Domain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD-M6PR Lumenal Domain Antibody

For research use only

Related Research Areas

Lysosomal Enzymes

Citations for Human CD-M6PR Lumenal Domain Antibody

Customer Reviews for Human CD-M6PR Lumenal Domain Antibody

There are currently no reviews for this product. Be the first to review Human CD-M6PR Lumenal Domain Antibody and earn rewards!

Have you used Human CD-M6PR Lumenal Domain Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies