CD14 occurs both as a secreted protein and as a GPI-linked membrane protein. It plays a critical role in lipopolysaccharide (LPS)-dependent signal transduction.
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Binding Assay, ELISA Capture, ELISA Microarray Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 50040
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571
Thr20-Cys352
Accession # P08571
Specificity
Detects human CD14 in sandwich ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD14 Antibody (50040)
Human CD14 ELISA Standard Curve
Recombinant Human CD14 (Catalog # 383-CD) was serially diluted and captured by Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3833) coated on a Clear Polystyrene Microplate (Catalog # DY990). Detection antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human CD14 Antibody (50040)
Human CD14 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB3833 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD14
Alternate Names
CD14
Gene Symbol
CD14
UniProt
Additional CD14 Products
Product Documents for Human CD14 Antibody (50040)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CD14 Antibody (50040)
For research use only
Citations for Human CD14 Antibody (50040)
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Customer Reviews for Human CD14 Antibody (50040) (2)
4.5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: Whole bloodSpecies: HumanVerified Customer | Posted 10/14/2021
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/17/2017This antibody was used to create a competitive ELISA with labeled recombinant CD14 as the competing molecule.
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Associated Pathways
