Human CD1c/BDCA-1 Antibody

Detection of Human CD1c by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD1c Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD1c at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.

Detection of CD1c by Immunohistochemistry

Co-localisation of human CD1c+ DC with ferroptotic PTEC in fibrotic kidney tissue.Representative serial section immunohistochemical (IHC) staining of control/non-fibrotic (left panel) and fibrotic (middle panel) kidney tissue probed for (A) aquaporin-1, (B) GPX4, (C) 4-HNE and (D) CD1c. Regions of CD1c+ DC co-localisation with aqupaporin-1+ PTEC displaying evidence of ferroptotic cell death (↓ GPX4 and ↑ 4-HNE) are highlighted with black arrows. Scale bars represent 60 µm. Quantitative analysis (positive pixel intensity/µm2 total area) of IHC staining in control and fibrotic tissue is presented (right panels). Symbols represent values for individual donor tissue. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. *P < 0.05, ***P < 0.001, Welch’s t test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36030251), licensed under a CC-BY license. Not internally tested by R&D Systems.