Human CD20 (Research Grade Rituximab Biosimilar) Antibody (Hu2) [Biotin]

Name: Human CD20 (Research Grade Rituximab Biosimilar) Antibody (Hu2) [Biotin]
Brand: R&D Systems
SKU: FAB9575B
Price: 389 USD
Availability: InStock

R&D Systems | Catalog # FAB9575B

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Product Documents

Recombinant Monoclonal Antibody For Research Use Only

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # Hu2

View all CD20 Primary Antibodies »

Product Specifications

Immunogen

Human CD20

Specificity

Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Scientific Data Images

Detection of MS4A1/CD20 in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.

HEK293 Human cell line transfected with (A) Human MS4A1/CD20 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human MS4A1/CD20 (Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

CD20 in Daudi Human Cell Line.

CD20 was detected in unfixed live Daudi human Burkitt's lymphoma cell line using Human Anti-Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Unfixed live Daudi human Burkitt's lymphoma cell line

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

Rituximab is a biosimilar directed against the extracellular domain of CD20. The biotinylated antibody can be used in flow cytometry or various assay format to measure CD20 expression and can be detected by conjugated streptavidin.