Human CD3 epsilon F(ab')2 Antibody (UCHT1R-FAB2-TEV/His)

R&D Systems | Catalog # MAB100R-FAB2

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # UCHT1R-FAB2-TEV/His
View all CD3 epsilon Primary Antibodies »

Product Specifications

Immunogen

Human thymocytes

Specificity

Detects human CD3 epsilon

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD3 epsilon F(ab')2 Antibody (UCHT1R-FAB2-TEV/His)

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry with anti-Mouse Secondary Antibody.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD3e FAB2 Recombinant Monoclonal Antibody (Catalog # MAB100R-FAB2) or (B) Mouse IgG1 isotype control antibody (MAB002), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). Staining was performed using our staining membrane proteins protocol.

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry with anti-His antibody.

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD3e FAB2 Recombinant Monoclonal Antibody (Catalog # MAB100R-FAB2) or (B) Mouse IgG1 isotype control antibody (MAB002), followed by PE-conjugated Anti-His Antibody (IC050P) and Mouse anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Staining was performed using our staining membrane proteins protocol.

Applications for Human CD3 epsilon F(ab')2 Antibody (UCHT1R-FAB2-TEV/His)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: PBMC lymphocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References

  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for Human CD3 epsilon F(ab')2 Antibody (UCHT1R-FAB2-TEV/His)

Certificate of Analysis

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Product Specific Notices for Human CD3 epsilon F(ab')2 Antibody (UCHT1R-FAB2-TEV/His)

For research use only

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