Human Cerberus 1 Antibody

R&D Systems | Catalog # AF1075

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cerberus 1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cerberus 1
Thy18-Ala267
Accession # O95813

Specificity

Detects human Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human DAN, recombinant mouse Gremlin, and recombinant chicken Caronte is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cerberus 1 Antibody

Detection of Human Cerberus 1 antibody by Western Blot.

Detection of Human Cerberus 1 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cerberus 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cerberus 1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Cerberus 1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human liver tissue

Reviewed Applications

Read 1 review rated 4 using AF1075 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cerberus 1

Cerberus 1 functions during development by binding to and antagonizing some members of the bone morphogenetic protein (BMP) family. Cerberus 1 is alternatively known as cerberus-related 1 and is a homolog of Xenopus cerberus.

Alternate Names

CER1, DAND4

Entrez Gene IDs

9350 (Human); 12622 (Mouse)

Gene Symbol

CER1

UniProt

O95813

Additional Cerberus 1 Products

Product Documents for Human Cerberus 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cerberus 1 Antibody

For research use only

Related Research Areas

BMP Family

Citations for Human Cerberus 1 Antibody

Customer Reviews for Human Cerberus 1 Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
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4 Stars
100%
3 Stars
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2 Stars
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1 Stars
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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 24176640
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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