Human CLEC2L Antibody (2326G)

R&D Systems | Catalog # MAB10204

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2326G
View all CLEC2L Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human CLEC2L protein
Ser90-Thr214
Accession # P0C7M8-1

Specificity

Detects human CLEC2L in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human CLEC2L Antibody (2326G)

Detection of CLEC2L in HEK293 Human Cell Line Transfected with Human CLEC2L and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with human CLEC2L and eGFP was stained with (A) Rabbit Anti-Human CLEC2L Monoclonal Antibody (Catalog # MAB2531) or (B) Rabbit IgG control antibody (MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CLEC2L Antibody (2326G)

Application
Recommended Usage

Flow Cytometry

0.5 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human CLEC2L and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC2L

C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.

References

  1. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
  2. Vogler, I. and A. Steinle (2011) J. Innate Immune. 3:227.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.
  5. Rosen, D.B. et al. (2008) J. Immunol. 180:6508.
  6. Lysenko, O. et al. (2013) PloS One. 8:e65345.

Long Name

C-type Lectin Domain Family 2, Member L

Alternate Names

C-type lectin domain family 2 member L, C-type lectin domain family 2, member L

Entrez Gene IDs

154790 (Human); 665180 (Mouse); 296985 (Rat)

Gene Symbol

CLEC2L

UniProt

P0C7M8-1

Additional CLEC2L Products

Product Documents for Human CLEC2L Antibody (2326G)

Certificate of Analysis

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Product Specific Notices for Human CLEC2L Antibody (2326G)

For research use only

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