CLEC3B (C-type lectin domain family 3-member B; also Tetranectin) is a 20 kDa, secreted member of the C type lectin superfamily. It is produced by multiple cell types including monocytes, neutrophils, fibroblasts, hepatocytes and various endocrine cells. Although named Tetranectin, CLEC3B is actually a nondisulfide linked homotrimer. It binds to plasminogen, sulfated polysaccharides, and fibrin. Human CLEC3B precursor is 202 amino acids (aa) in length. It contains a signal sequence (aa 1-21), a coiled coil region that mediates trimerization (aa 26-52) and a C type lectin domain (aa 77-198). CLEC3B contains O-linked glycosylation. Mature human CLEC3B is 81% aa identical to mouse CLEC3B.
Human CLEC3B/Tetranectin Antibody (805035)
R&D Systems | Catalog # MAB5170
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Blockade of Receptor-ligand Interaction, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 805035
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC3B/Tetranectin
Glu22-Val202
Accession # P05452
Glu22-Val202
Accession # P05452
Specificity
Detects human CLEC3B/Tetranectin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CLEC3B/Tetranectin Antibody (805035)
Detection of Human CLEC3B/Tetranectin by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC3B/Tetranectin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CLEC3B/Tetranectin by Simple WesternTM.
Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. Specific bands were detected for CLEC3B/Tetranectin at approximately 32 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human CLEC3B/Tetranectin Antibody (805035)
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
5 µg/mL
Sample: Human plasma
Sample: Human plasma
Western Blot
0.5 µg/mL
Sample: Human plasma
Sample: Human plasma
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CLEC3B/Tetranectin
Long Name
C-type Lectin-like Receptor 3B
Alternate Names
TETN, Tetranectin, TNA
Gene Symbol
CLEC3B
UniProt
Additional CLEC3B/Tetranectin Products
Product Documents for Human CLEC3B/Tetranectin Antibody (805035)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CLEC3B/Tetranectin Antibody (805035)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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