Human Collagen IV alpha 1 Antibody (577238)

R&D Systems | Catalog # MAB6308

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 577238
View all Collagen IV alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Collagen IV alpha 1
Ser1441-Thr1669
Accession # P02462

Specificity

Detects human Collagen IV alpha 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Collagen I alpha 1, III alpha 1, XIII alpha 1v4, XXIII alpha 1, or recombinant mouse Collagen XXV alpha 1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Collagen IV alpha 1 Antibody (577238)

Collagen IV a1 antibody in T98G Human Cell Line by Immunocytochemistry (ICC).

Collagen IV alpha 1 in T98G Human Cell Line.

Collagen IV a1 was detected in immersion fixed T98G human glioblastoma cell line using Mouse Anti-Human Collagen IV alpha 1 Monoclonal Antibody (Catalog # MAB6308) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to perinuclear areas and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Collagen IV a1 antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC).

Collagen IV alpha 1 in THP‑1 Human Cell Line.

Collagen IV a1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Collagen IV a1 Monoclonal Antibody (Catalog # MAB6308) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Collagen IV alpha 1 Antibody (577238)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed T98G human glioblastoma cell line and THP-1 human acute monocytic leukemia cell line

Reviewed Applications

Read 1 review rated 5 using MAB6308 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collagen IV alpha 1

COL4A1 (collagen IV alpha 1) is a 185 kDa member of the type IV collagen family.  It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells.  Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix.  This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane.  Mature human COL4A1 is 1642 amino acids (aa) in length.  It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669).  Multiple splice forms exist.  One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669.  Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.

Alternate Names

Arresten, COL4A1

Entrez Gene IDs

1282 (Human); 12826 (Mouse); 290905 (Rat)

Gene Symbol

COL4A1

UniProt

P02462 (Human)

Additional Collagen IV alpha 1 Products

Product Documents for Human Collagen IV alpha 1 Antibody (577238)

Certificate of Analysis

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Product Specific Notices for Human Collagen IV alpha 1 Antibody (577238)

For research use only

Citations for Human Collagen IV alpha 1 Antibody (577238)

Customer Reviews for Human Collagen IV alpha 1 Antibody (577238) (1)

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  • Human Collagen IV  alpha 1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 12/07/2016
    Human Collagen IV alpha 1 Antibody (577238) MAB6308

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