Human Collagen IV alpha 1 Antibody

R&D Systems | Catalog # AF6308

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Collagen IV alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Collagen IV alpha 1
Ser1441-Thr1669
Accession # P02462

Specificity

Detects human Collagen IV alpha 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse COL13A1, recombinant human (rh) COL25A1 and rhCOL23A1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Collagen IV alpha 1 Antibody

Detection of Human Collagen IV a1 antibody by Western Blot.

Detection of Human Collagen IV alpha 1 by Western Blot.

Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 1 µg/mL of Human Collagen IV a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6308) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Collagen IV a1 at approximately 95 kDa (as indicated) ref 1. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Collagen IV  alpha 1 in Human Kidney.

Collagen IV  alpha 1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Sheep Anti-Human Collagen IV  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6308) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Collagen IV alpha 1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Kidney

Western Blot

1 µg/mL
Sample: Human placenta tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collagen IV alpha 1

COL4A1 (collagen 4-alpha 1) is a calculated 185 kDa (ref. 1) member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes, and endothelial cells. Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642 amino acids (aa) in length (SwissProt #:P02462). It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C‑terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669). Multiple splice forms exist. One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958‑1669. Over aa 1441-1669, human COL4A1 shares 97% aa sequence identity to mouse COL4A1. AF6308 detects a 95 kDa band, endogenous COL4A1 by Westen blot in human placenta tissue sample, which is consistent with the current literature (ref. 1)

References

  1. Protein Science (2006), 15:2805.

Alternate Names

Arresten, COL4A1

Entrez Gene IDs

1282 (Human); 12826 (Mouse); 290905 (Rat)

Gene Symbol

COL4A1

UniProt

P02462 (Human)

Additional Collagen IV alpha 1 Products

Product Documents for Human Collagen IV alpha 1 Antibody

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Product Specific Notices for Human Collagen IV alpha 1 Antibody

For research use only

Citations for Human Collagen IV alpha 1 Antibody

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Protocols

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