Human Collagen XIII alpha 1 Antibody

R&D Systems | Catalog # AF6346

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Collagen XIII alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Collagen XIII
Glu109-Leu668
Accession # NP_542992

Specificity

Detects human Collagen XIII in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse COL13A1 and less than 1% cross-reactivity with recombinant human (rh) COL23A1 and rhCOL25A1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Collagen XIII alpha 1 Antibody

Detection of Human Collagen XIII a1 antibody by Western Blot.

Detection of Human Collagen XIII alpha 1 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF Membrane was probed with 1 µg/mL of Human Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6346) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Two specific bands were detected for Collagen XIII a1 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Collagen XIII alpha 1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collagen XIII alpha 1

COL13A1 (collagen 13-alpha 1) is a 95 kDa member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the integrin alpha 1 subunit, nidogen-2, and fibronectin. Human COL13A1 is 717 amino acids (aa) in length (SwissProt #:Q5TAT6). It has an N‑terminal 44 amino acid cytoplasmic region plus a 656 amino acid extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, 217-269, 442-463, and 700-717) interspersed among three collagenous (COL) domains (aa 122-216, 270-441, and 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90 kDa soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, 551-565, and 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1.

Alternate Names

COL13A1

Entrez Gene IDs

1305 (Human); 12817 (Mouse); 499431 (Rat)

Gene Symbol

COL13A1

UniProt

NP_542992

Additional Collagen XIII alpha 1 Products

Product Documents for Human Collagen XIII alpha 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Collagen XIII alpha 1 Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Human Collagen XIII alpha 1 Antibody

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Associated Pathways

Articular Cartilage Extracellular Matrix
Articular Cartilage Extracellular Matrix Thumbnail