Human CRELD1 Antibody (842314)

R&D Systems | Catalog # MAB3128

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 842314
View all CRELD1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRELD-1
Gln30-Glu362
Accession # Q96HD1

Specificity

Detects human CRELD-1 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CRELD1 Antibody (842314)

Cell Adhesion Mediated by CRELD1 and Neutralization by Human CRELD1 Antibody.

Cell Adhesion Mediated by CRELD1 and Neutralization by Human CRELD1 Antibody.

Recombinant Human CRELD1 (Catalog # 3128-CR), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CRELD1 (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CRELD1 Monoclonal Antibody (Catalog # MAB3128). The ND50 is typically 0.15-0.75 µg/mL.

Applications for Human CRELD1 Antibody (842314)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize CRELD1-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.15-0.75 μg/mL in the presence of 2 μg/mL Recombinant Human CRELD1.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRELD1

CRELD1, also known as cirrin, is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain with two EGF-like repeats, 2 transmembrane domains separated by a very short cytoplasmic loop, and a short extracellular C-terminal domain. An alternatively spliced isoform with a unique C-terminus that lacks the transmembrane domains has also been cloned. Missense mutations in CRELD1 have been associated with atrioventricular septal defects. The N-terminal extracellular domain of human and mouse CRELD1 share 92% aa sequence identity.

Long Name

Cysteine-rich Protein With EGF-like Domains 1

Alternate Names

AVSD2, CIRRIN

Entrez Gene IDs

78987 (Human); 171508 (Mouse); 312638 (Rat)

Gene Symbol

CRELD1

UniProt

Q96HD1

Additional CRELD1 Products

Product Documents for Human CRELD1 Antibody (842314)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CRELD1 Antibody (842314)

For research use only

Citations for Human CRELD1 Antibody (842314)

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