Human CTRP5/C1qTNF5 Antibody (332923)

R&D Systems | Catalog # MAB3167

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 332923
View all CTRP5/C1qTNF5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CTRP5/C1qTNF5
Ser16-Ala243 (Gln44Arg)
Accession # Q9BXJ0

Specificity

Detects human CTRP5/C1qTNF5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CTRP1 or rhCTRP4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CTRP5/C1qTNF5 Antibody (332923)

CTRP5/C1qTNF5 antibody in Human Liver by Immunohistochemistry (IHC-P).

CTRP5/C1qTNF5 in Human Liver.

CTRP5/C1qTNF5 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CTRP5/C1qTNF5 Monoclonal Antibody (Catalog # MAB3167) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of CTRP5/C1qTNF5 antibody in A172 Human Cell Line antibody by Flow Cytometry.

Detection of CTRP5/C1qTNF5 in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Mouse Anti-Human CTRP5/C1qTNF5 Monoclonal Antibody (Catalog # MAB3167, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human CTRP5/C1qTNF5 Antibody (332923)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) 

Western Blot

1 µg/mL
Sample: Recombinant Human CTRP5/C1qTNF5

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CTRP5/C1qTNF5

CTRP5, also known as C1qTNF5, belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF‑related protein family. All family members share a modular organization comprising an N‑terminal signal peptide, a short variable region, a collagenous domain and a C‑terminal globular domain. CTRP proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. Human and mouse CTRP5 share 94% amino acid sequence identity.

Long Name

C1q And Tumor Necrosis Factor Related Protein 5

Alternate Names

C1qTNF5

Entrez Gene IDs

114902 (Human); 235312 (Mouse)

Gene Symbol

C1QTNF5

UniProt

Q9BXJ0

Additional CTRP5/C1qTNF5 Products

Product Documents for Human CTRP5/C1qTNF5 Antibody (332923)

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Product Specific Notices for Human CTRP5/C1qTNF5 Antibody (332923)

For research use only

Citations for Human CTRP5/C1qTNF5 Antibody (332923)

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Protocols

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