Human CXCL9/MIG Antibody (49106)

R&D Systems | Catalog # MAB392

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunodepletion, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 49106
View all CXCL9/MIG Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL9/MIG
Thr23-Thr125
Accession # Q07325

Specificity

Detects human CXCL9/MIG in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant mouse (rm) CXCL9, recombinant human CXCL10.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCL9/MIG Antibody (49106)

Chemotaxis Induced by CXCL9/MIG and Neutralization by Human CXCL9/MIG Antibody.

Chemotaxis Induced by CXCL9/MIG and Neutralization by Human CXCL9/MIG Antibody.

Recombinant Human CXCL9/MIG (Catalog # 392-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL9/MIG (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392). The ND50 is typically 0.5-4 µg/mL.

CXCL9/MIG in THP‑1 Human Cell Line.

CXCL9/MIG was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line treated with IFN gamma (positive staining) and THP‑1 human acute monocytic leukemia cell line (untreated; negative staining) using Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Human CXCL9 / MIG ELISA Standard Curve

Human CXCL9 / MIG ELISA Standard Curve

Recombinant Human CXCL9/MIG (Catalog # 392-MG) was serially diluted and captured by Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF392) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of CXCL9/MIG by Immunohistochemistry

Detection of CXCL9/MIG by Immunohistochemistry

CCR2 ligands are secreted but do not bind to HUVECs.a Culture medium collected from the upper and lower wells of transwells with HUVEC monolayers either not stimulated (NS) or stimulated with TNF-alpha + IFN-gamma were assayed for CCL2, CCL7, and CCL8 by ELISA (n = 3 separate experiments). Each symbol shows data from one experiment, with assays done in duplicate, and bars indicate means ± SEM. b Confocal microscopy images at × 40 magnification of permeabilized and non-permeabilized TNF-alpha + IFN-gamma -stimulated HUVECs immunostained for CCL2, CCL7, CCL8, CCL5, CCL20, and CXCL9 (green) and stained using phalloidin for polymerized actin (magenta) and DAPI for nuclei (blue). The scale bars indicate 10 µm. Images are representative of three experiments. c Biotinylated CCL5 (0.1 μM) and CCL2 (1 μM) were incubated with CHO-K1 cells and the heparan-sulfate deficient cell line, D-677. Chemokine binding was detected using streptavidin-phycoerythrin (PE) and flow cytometry. Data shown are representative of three separate experiments. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-025-57002-6), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCL9/MIG Antibody (49106)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line treated with IFN gamma

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: THP-1 cells treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human CXCL9/MIG (Catalog # 392-MG)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 0.5-4 µg/mL in the presence of 0.25 µg/mL Recombinant Human CXCL9/MIG.

Human CXCL9/MIG Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CXCL9/MIG Biotinylated Antibody (Catalog # BAF392)
  • Standard: Recombinant Human CXCL9/MIG Protein (Catalog # 392-MG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using MAB392 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL9/MIG

CXCL9, a member of the alpha  subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide that is cleaved to yield a 103 aa residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic aa residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. The E. coli-expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.

References

  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Liao, F. et al. (1995) J. Exp. Med. 182:1301.
  3. Vanguri, P. (1995) J. Neuroimmunol. 56:35.

Alternate Names

MIG

Entrez Gene IDs

4283 (Human); 17329 (Mouse); 246759 (Rat)

Gene Symbol

CXCL9

UniProt

Q07325

Additional CXCL9/MIG Products

Product Documents for Human CXCL9/MIG Antibody (49106)

Certificate of Analysis

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Product Specific Notices for Human CXCL9/MIG Antibody (49106)

For research use only

Citations for Human CXCL9/MIG Antibody (49106)

Customer Reviews for Human CXCL9/MIG Antibody (49106) (3)

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Showing  1 - 3 of 3 reviews Showing All
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  • Human CXCL9/MIG Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Primate
    Verified Customer | Posted 11/14/2022
    Worked as a capture antibody in our non-human primate ELISA
    Human CXCL9/MIG Antibody (49106) MAB392
  • Human CXCL9/MIG Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: macrophages
    Species: Human
    Verified Customer | Posted 09/04/2021
    CXCL9 antibody showed positive macrophages in hepatitis liver tissue.
    Human CXCL9/MIG Antibody (49106) MAB392
  • Human CXCL9/MIG Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017
    This antibody was used as a capture in a sandwich ELISA for MIG. The detection antibody was BAM392. The standard was 392-MG-010. Sensitivity was around 5 pg/ml.
    Human CXCL9/MIG Antibody (49106) MAB392

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