Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody

R&D Systems | Catalog # AF5546

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rabbit

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cytosolic Sulfotransferase 1A1/SULT1A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1A1/SULT1A1
Glu2-Leu295
Accession # P50225

Specificity

Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SULT1E1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody

Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 antibody by Western Blot.

Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 antibody by Simple WesternTM.

Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Cytosolic Sulfotransferase 1A1/SULT1A1 (Catalog # 5546-ST), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: Human liver tissue

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line and human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic Sulfotransferase 1A1/SULT1A1

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity (2). The enzyme is more specific for sulfonation on catecholamines, phenolic drugs and neurotransmitters (3). Because of its ability to modify diverse promutagen and procarcinogen xenobiotics, it is implicated in a range of cancers (2). The crystal structure of the enzyme reveals that its active site is flexible and can accommodate diverse hydrophobic substrates with varying sizes, shapes and flexibility (4).

References

  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Wilborn, T.W. et al. (1993) Mol. Pharm. 43:70.
  4. Gamage, N. U. et al. (2003) J. Biol. Chem. 278:7655.
  5. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  6. MacRae, I.J. et al. (2000) Biochemistry 39: 1613.
  7. Wu, Z.L. et al. (2002) Faseb J. 16:539.

Alternate Names

P-PST, PST, ST1A3, STP1, SULT1A1, TSPST1

Entrez Gene IDs

6817 (Human); 20887 (Mouse); 83783 (Rat)

Gene Symbol

SULT1A1

UniProt

P50225

Additional Cytosolic Sulfotransferase 1A1/SULT1A1 Products

Product Documents for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody

For research use only

Related Research Areas

Sulfation Regulators

Citations for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody

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