Human DAP10/HCST Antibody (982129)

R&D Systems | Catalog # MAB9786

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 982129
View all DAP10/HCST Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human DAP10/HCST
Accession # Q9UBK5

Specificity

Detects human DAP10/HCST in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human DAP10/HCST Antibody (982129)

Detection of DAP10/HCST antibody in U937 cell line antibody by Flow Cytometry.

Detection of DAP10/HCST in U937 cell line by Flow Cytometry.

Human U937 histiocytic lymphoma cell line was stained with Mouse Anti-Human DAP10/HCST Monoclonal Antibody (Catalog # MAB9786, filled histogram) or Mouse IgG2A isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human DAP10/HCST Antibody (982129)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: U937 Human Histiocytic Lymphoma Cell Line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DAP10/HCST

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.

Alternate Names

HCST, KAP10, Kinase Assoc Protein, PIK3AP

Entrez Gene IDs

10870 (Human); 23900 (Mouse); 474146 (Rat)

Gene Symbol

HCST

UniProt

Q9UBK5

Additional DAP10/HCST Products

Product Documents for Human DAP10/HCST Antibody (982129)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DAP10/HCST Antibody (982129)

For research use only

Citations for Human DAP10/HCST Antibody (982129)

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