DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.
Human DAP12 Antibody (406209)
R&D Systems | Catalog # MAB52401
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 406209
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Product Specifications
Immunogen
Human Dap12 synthetic peptide
Accession # O43914
Accession # O43914
Specificity
Detects human DAP12 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human DAP12 Antibody (406209)
Detection of DAP12 in Human CD56+Natural Killer Cells by Flow Cytometry.
Human PBMCs were stained with (A) Human DAP12 Monoclonal Antibody (Catalog # MAB52401) or (B) isotype control antibody (MAB003), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B) and Rabbit anti-human CD56 PE-conjugated Monoclonal Antibody (FAB24086P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (1X) (FC005).Applications for Human DAP12 Antibody (406209)
Application
Recommended Usage
Flow Cytometry
0.25 µg/mL
Sample: Human PBMCs
Sample: Human PBMCs
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DAP12
Long Name
DNAX-activation Protein 12
Alternate Names
KARAP, TYROBP
Gene Symbol
TYROBP
UniProt
Additional DAP12 Products
Product Documents for Human DAP12 Antibody (406209)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human DAP12 Antibody (406209)
For research use only
Citations for Human DAP12 Antibody (406209)
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