Human DEC-205/CD205 Antibody (523218)

R&D Systems | Catalog # MAB20471

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 523218
View all DEC-205/CD205 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DEC‑205/CD205
Cys216-Cys501
Accession # O60449

Specificity

Detects human DEC‑205/CD205 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse DEC-205 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human DEC-205/CD205 Antibody (523218)

Detection of Human DEC‑205/CD205 antibody by Western Blot.

Detection of Human DEC‑205/CD205 by Western Blot.

Western blot shows lysates of in vitro differentiated human immature myeloid dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DEC-205/CD205 Monoclonal Antibody (Catalog # MAB20471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEC-205/CD205 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Detection of Human DEC‑205/CD205 by Western Blot.

Western blot shows lysates of human thymus. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DEC‑205/CD205 Monoclonal Antibody (Catalog # MAB20471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for DEC‑205/CD205 at approximately 250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Applications for Human DEC-205/CD205 Antibody (523218)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: In vitro differentiated human immature myeloid dendritic cells and human thymus

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti-human DEC-205 antibody contains the first two C-type lectin domains.

Alternate Names

CD205, DEC205, Ly75

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Gene Symbol

LY75

UniProt

O60449

Additional DEC-205/CD205 Products

Product Documents for Human DEC-205/CD205 Antibody (523218)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DEC-205/CD205 Antibody (523218)

For research use only

Customer Reviews for Human DEC-205/CD205 Antibody (523218)

There are currently no reviews for this product. Be the first to review Human DEC-205/CD205 Antibody (523218) and earn rewards!

Have you used Human DEC-205/CD205 Antibody (523218)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

Dendritic Cell Lineage Development Pathways
Dendritic Cell Lineage Development Pathway Thumbnail