Human DNER Antibody [Biotin]

R&D Systems | Catalog # BAF3646

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all DNER Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DNER
Arg29-His637
Accession # Q8NFT8

Specificity

Detects human DNER in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DNER Antibody [Biotin]

Detection of Human DNER antibody by Western Blot.

Detection of Human DNER by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3646) followed by streptavidin-HRP. A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DNER Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)

Western Blot

0.2 µg/mL
Sample: Human brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNER

DNER (delta and notch-like EGF-related receptor; also BET) is a 120-150 kDa type I transmembrane glycoprotein that is reminescent of notch ligands such as Delta and Jagged. It is found on neuron dendrites and cell bodies but not on axons. Human DNER is 703 amino acids (aa) in length, and contains a 606 aa extracellular domain (ECD) (aa 35-640) plus a 76 aa cytoplasmic region. The ECD contains nine consecutive EGF-like domains, followed by one follistatin-like domain and a tenth EGF-like domain. DNER binding to notch-1 on glia induces morphological differentiation. Human DNER ECD is 89% aa identical to mouse DNER ECD.

Long Name

Delta/Notch-like EGF Repeat-containing Transmembrane

Alternate Names

BET, delta and Notch-like epidermal growth factor-related receptor, delta/notch-like EGF repeat containing, delta-notch-like EGF repeat-containing transmembrane, H_NH0150O02.1, UNQ26, WUGSC:H_NH0150O02.1

Entrez Gene IDs

92737 (Human); 227325 (Mouse)

Gene Symbol

DNER

UniProt

Q8NFT8

Additional DNER Products

Product Documents for Human DNER Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DNER Antibody [Biotin]

For research use only

Related Research Areas

Notch Pathway

Citations for Human DNER Antibody [Biotin]

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail