Human DSCAM Long Isoform Antibody (399212)

R&D Systems | Catalog # MAB36661

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 399212
View all DSCAM Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DSCAM Long Isoform
Glu18-Met1595
Accession # O60469

Specificity

Detects human DSCAM Long Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human DSCAM-L1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DSCAM Long Isoform Antibody (399212)

Detection of Recombinant Human DSCAM antibody by Western Blot.

Detection of Recombinant Human DSCAM by Western Blot.

Western blot shows 25 ng of Recombinant Human DSCAM (Catalog # 3666-DS) and Recombinant Human DSCAM-L1 (Catalog # 3315-DL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human DSCAM Long Isoform Monoclonal Antibody (Catalog # MAB36661) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DSCAM at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
DSCAM antibody in A172 Human Cell Line by Immunocytochemistry (ICC).

DSCAM in A172 Human Cell Line.

DSCAM was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human DSCAM Long Isoform Monoclonal Antibody (Catalog # MAB36661) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse Human DSCAM Long Isoform Antibody by Western Blot

Detection of Mouse Human DSCAM Long Isoform Antibody by Western Blot

The C-terminus of DSCAM is not required for protein stability or localization. (C) Western blots of DSCAM protein immunoprecipitated from neonatal brains showed no change in the size or amount of DSCAM in Dscam∆C/∆C mutants. The antibody specificity is confirmed by the lack of signal from Dscam-/- brains. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27637097), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human DSCAM Long Isoform Antibody (399212)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human DSCAM (Catalog # 3666-DS)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DSCAM

Down syndrome cell adhesion molecule (DSCAM) is a 220 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily (1). Human DSCAM, which maps to a Down syndrome region of chromosome 21q22.2‑22.3, is synthesized as a 2012 amino acid (aa) precursor that contains a 17 aa signal sequence, a 1578 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 396 aa cytoplasmic tail. The ECD contains ten Ig-like C2-type domains, six fibronectin type III domains, and 16 potential sites for N-linked glycosylation. Splicing variants lead to a second, shorter isoform, which has a ten aa substitution for aa 1562‑1571 in the longer isoform, and a deletion of residues corresponding to aa 1572‑2012 in the longer isoform. Human mature DSCAM is 98% aa identical to mature mouse and rat DSCAM. Studies on mice have shown that DSCAM is expressed widely in the developing nervous system (1, 2). More recent studies indicate that DSCAM plays an important role in neurite arborization, cell body spacing, and lamina-specific synaptic targeting in vertebrate retina (2‑4). DSCAM directly binds to cytoplasmic Pak1 and stimulates Pak1 phosphorylation and activity (5). In addition, DSCAM activates both JNK and p38 MAP kinases, and expression of the cytoplasmic domain of DSCAM induces a morphological change in cultured cells that is JNK-dependent (5). Thus, it appears that DSCAM signals through Pak1 and functions in axon guidance. Furthermore, DSCAM, in collaboration with DCC, interacts with Netrin-1 and is a receptor required for Netrin-dependent commissural axon outgrowth and pathfinding (2, 6).

References

  1. Yamakawa, K. et al. (1998) Hum. Mol. Genet. 7:227.
  2. Liu, G. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:2951.
  3. Fuerst, P.G. et al. (2008) Nature 451:470.
  4. Yamagata, M. and J.R. Sanes (2008) Nature 451:465.
  5. Li, W. and K.-L. Guan (2004) J. Biol. Chem. 279:32824.
  6. Ly, A. et al. (2008) Cell 133:11241.

Long Name

Down Syndrome Cell Adhesion Molecule

Alternate Names

CHD2-42, CHD2-52

Entrez Gene IDs

1826 (Human); 13508 (Mouse)

Gene Symbol

DSCAM

UniProt

O60469

Additional DSCAM Products

Product Documents for Human DSCAM Long Isoform Antibody (399212)

Certificate of Analysis

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Product Specific Notices for Human DSCAM Long Isoform Antibody (399212)

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

Citations for Human DSCAM Long Isoform Antibody (399212)

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Protocols

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