Human EGR1 Antibody

Detection of EGR1 in SK‑BR‑3 Human Cell Line.

EGR1 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Goat Anti-Human EGR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2818) at 5 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of EGR1 by Western Blot

Gene ontology classification of differentially expressed genes regulated by silencing of heparanase gene (HPSE) expression. (A) Gene ontology (GO) analysis of up‐ and (B) down‐regulated genes after silencing of HPSE in MDA‐MB‐435s cells by terms of biological process and cellular component. X‐axis indicates functional fold enrichment calculated by binomial test, P < 0.01. (C) Listing of an array of 28 pro‐apoptotic genes classified by GO term positive regulation of cell death and apoptotic process. Y‐axis indicates fold change comparing HPSE silenced cells with control cells. Dashed line indicates 1.5‐fold change. (D) Validation of expression of the 28 pro‐apoptotic genes by real‐time PCR. n = 3 biological repeats, * indicates the selected genes for further validation by Western blots. Dashed line indicates 1.5‐fold change. (E) Validation of up‐regulation of selected genes including CYR61, EGR1 and TNFRSF12A on protein level by Western blots. N = 3 biological repeats, representative blots are shown Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31044520), licensed under a CC-BY license. Not internally tested by R&D Systems.