Human Ephrin-A5 Antibody

R&D Systems | Catalog # AF3743

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Ephrin-A5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Ephrin-A5
Gln21-Asn203
Accession # P52803

Specificity

Detects human Ephrin-A5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, rmEphrin-A2, recombinant human (rh) Ephrin-A3, rhEphrin-A4, rhEphrin-B3, rmEphrin-A4, rmEphrin-B1 and rmEphrin-B2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Ephrin-A5 Antibody

Ephrin-A5 antibody in Human Melanoma by Immunohistochemistry (IHC-P).

Ephrin‑A5 in Human Melanoma.

Ephrin-A5 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human Ephrin-A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3743) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Ephrin-A5 by Immunohistochemistry

Detection of Ephrin-A5 by Immunohistochemistry

Increased Notch and Ephrin signaling in metabolically inflexible human beta cells.(A) scRNA-Seq of islets from nondiabetic and T2D patients, with beta cells subclustered by metabolic pathways to define healthy (H beta ) and metabolically inflexible (MI beta ) as per a previous report (48). tSNE, t-distributed stochastic neighbor embedding. (B) Violin plots of RBPJ activity in H beta and MI beta cell populations. (C) GSEA of EPH-Ephrin signaling in DEGs of MI beta versus H beta populations. NES, normalized enrichment score. (D) Average activity of EPH-Ephrin signaling components in H beta and MI beta populations. (E) RBPJ activity as compared with EPH-Ephrin signaling components, with each dot representing a single H beta (black) and MI beta (red) cell. Extended figure with all EPH-Ephrin signaling components and Pearson’s r values in Supplemental Figure 11A. (F) Representative images of pancreatic sections stained with antibodies against EFNA5 and insulin from nondiabetic and T2D patients. Extended figure with additional staining in Supplemental Figure 12B. Scale bars: 20 μm. Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/157694), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Ephrin-A5 by Immunohistochemistry

Detection of Ephrin-A5 by Immunohistochemistry

RNA-Seq of beta -tetO-NICD islets reveals potential morphogenetic effectors.(A) Volcano plot showing differentially expressed genes (DEGs, adjusted P < 0.05) in islets from beta -tetO-NICD and Cre+ controls after 8 weeks’ Dox. (B) Downregulated and (C) upregulated biological processes in beta -tetO-NICD islets, after Gene Ontology enrichment analysis using Enrichr. (D) Heatmap of representative DEGs across samples, with log2-normalized gene expression value shown. Efna5, Ephrin A5. (E) STRING network analysis of upregulated DEGs in beta -tetO-NICD islets showing enrichment analysis of processes associated with the network, highlighting processes associated with tissue morphogenesis. (F) Absolute quantitation, relative to invariant control Ppia, of genes encoding Ephrin ligands in islets isolated from beta -tetO-NICD and control mice after 8 weeks’ Dox (n = 5–6 mice/group). (G) Representative images of pancreatic sections stained with antibodies against Efna5 and insulin from beta -tetO-NICD and Cre– controls after 8 weeks’ Dox. Scale bars: 20 μm. All data are shown with group means; *, P < 0.05, **, P < 0.01, ***, P < 0.001, ****, P < 0.0001 by 2-tailed t test. Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/157694), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Ephrin-A5 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human Ephrin-A5 Fc Chimera (Catalog # 374-EA)

Reviewed Applications

Read 3 reviews rated 4 using AF3743 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A5

Ephrin-A5, also known as AL-1, RAGS, LERK-7, and EFL-5 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A5 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of murine and human Ephrin-A5 share 99% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Alternate Names

AL-1, EFL-5, EFNA5, EphrinA5, LERK-7, RAGS

Entrez Gene IDs

1946 (Human); 13640 (Mouse)

Gene Symbol

EFNA5

UniProt

P52803

Additional Ephrin-A5 Products

Product Documents for Human Ephrin-A5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Ephrin-A5 Antibody

For research use only

Related Research Areas

Ephrin Ligands and Eph Receptors

Citations for Human Ephrin-A5 Antibody

Customer Reviews for Human Ephrin-A5 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Ephrin-A5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HT-29 colon cancer cell line and T84 human colon carcinoma cell line
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human Ephrin-A5 Antibody AF3743
  • Human Ephrin-A5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: DA3 mouse myeloma cell line
    Species: Mouse
    Verified Customer | Posted 02/18/2019
    Human Ephrin-A5 Antibody AF3743
  • Human Ephrin-A5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Capan-1 human pancreatic adenocarcinoma cell line and AR42J human acinar pancreatic cancer cell line
    Species: Human
    Verified Customer | Posted 10/17/2018
    Human Ephrin-A5 Antibody AF3743

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