Estrogen Receptor beta (ERβ,NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17β-estradiol. ERβ is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ERβ in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ERβ has been postulated to act primarily as a modulator of ERα function. ER&beta has been shown to form homodimers as well as heterodimers with ERα. Both ERα and ERβ can give rise to numerous isoforms.
Human ER beta/NR3A2 Antibody (PPZ0506)
R&D Systems | Catalog # PP-PPZ0506-00
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Immunohistochemistry, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PPZ0506
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Product Specifications
Immunogen
Human ER beta/NR3A2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human ER beta/NR3A2 Antibody (PPZ0506)
Detection of ER beta/NR3A2 by Immunohistochemistry
Female reproductive tissues of Esr2-depleted mice are negative for estrogen receptor beta (ER beta ) expression. Ovaries (A) and uterine tissue (B) of Esr2 knockout (Esr2−/−) and wild-type (Esr2+/+) animals from the same mouse line were stained with PPZ0506 antibody or with isotype-specific IgG2b negative control. Only granulosa cells (Gr) in Esr2+/+ ovaries strongly express ER beta, whereas in Esr2−/− ovary and uterine tissue no specific nuclear staining was observed. Specific staining is shown by arrowheads in Esr2+/+ and # indicates diffuse non-specific background staining. Scale bar 250 µm (100×) or 50 µm (400×). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2227-9059/10/12/3100), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ER beta/NR3A2 by Immunohistochemistry
Female reproductive tissues of Esr2-depleted mice are negative for estrogen receptor beta (ER beta ) expression. Ovaries (A) and uterine tissue (B) of Esr2 knockout (Esr2−/−) and wild-type (Esr2+/+) animals from the same mouse line were stained with PPZ0506 antibody or with isotype-specific IgG2b negative control. Only granulosa cells (Gr) in Esr2+/+ ovaries strongly express ER beta, whereas in Esr2−/− ovary and uterine tissue no specific nuclear staining was observed. Specific staining is shown by arrowheads in Esr2+/+ and # indicates diffuse non-specific background staining. Scale bar 250 µm (100×) or 50 µm (400×). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2227-9059/10/12/3100), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human ER beta/NR3A2 Antibody (PPZ0506)
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using PP-PPZ0506-00 in the following applications:
Formulation, Preparation, and Storage
Formulation
A liquid formulation in physiologic saline with 0.1% NaN<sub>3</sub>.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: ER beta/NR3A2
Long Name
Estrogen Receptor beta
Alternate Names
ESR2, NR3A2
Entrez Gene IDs
2100 (Human)
Gene Symbol
ESR2
Additional ER beta/NR3A2 Products
Product Documents for Human ER beta/NR3A2 Antibody (PPZ0506)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human ER beta/NR3A2 Antibody (PPZ0506)
For research use only
Related Research Areas
Citations for Human ER beta/NR3A2 Antibody (PPZ0506)
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Customer Reviews for Human ER beta/NR3A2 Antibody (PPZ0506) (2)
5 out of 5
2 Customer Ratings
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Application: ImmunohistochemistrySample Tested: Ovary tissueSpecies: HumanVerified Customer | Posted 09/16/2025heating pretreatment, x200 primary antibody
Bio-Techne ResponseThis review reflects a new species or application tested on a primary antibody. -
Application: ImmunohistochemistrySample Tested: Cancer Tissue and Normal tissueSpecies: HumanVerified Customer | Posted 08/15/2017The present antibody proved excellent for IHC (1:600 dilution) of formalin-fixed paraffin-embedded tissues and cells, Western blotting (1:1000) and IP as determined by comparing results from ERbeta-positive cells and ERbeta-negative cells, and corroborated by mass spectrometry. It was tested on a wide range of human normal tissues and cancer, and correlated to mRNA levels (RNA-seq). It stained the nucleus of granulosa cells of the normal ovary, testis, adrenal gland, tonsil, and lymphocytes in various tissues; tumor cells of granulosa cell tumors and a subset of malignant melanoma. See full article at Nature Communications (Andersson et al, 2017): https://www.nature.com/articles/ncomms15840
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
