Human FAM3D Antibody

R&D Systems | Catalog # AF2869

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FAM3D Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FAM3D
Trp39-Phe224
Accession # Q96BQ1

Specificity

Detects human FAM3D in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse FAM3D is observed and 5% cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, and rhFAM3C is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FAM3D Antibody

Detection of FAM3D by Western Blot

Detection of FAM3D by Western Blot

Correction of the V600E mutation in BRAF leads to upregulation of WNT antagonist genesmRNA expression (2log) of WNT antagonist (A) FAM3D in HUB040 and NC versus BC1 and BC2.(B) Top differentially expressed genes (HUB040 and NC < BC1 and BC2) sorted on 2log difference (t-test, FDR corrected). mRNA expression of (C) DKK1, (D) CDH1, (E) FAM3D, and (F) SOSTDC1 in CRC TCGA dataset (BRAF-M, n = 57; BRAF-wt n = 467), and colon cancer dataset Marisa et al. (BRAF-M, n = 51; BRAF-wt n = 461).(G) Immunoblot analysis of FAM3D in HUB040, NC, BC1 and BC2. Unpaired t-test ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. CRC, colorectal cancer; TCGA, The Cancer Genome Atlas. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40687798), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FAM3D by Western Blot

Detection of FAM3D by Western Blot

Correction of the V600E mutation in BRAF leads to upregulation of WNT antagonist genesmRNA expression (2log) of WNT antagonist (A) FAM3D in HUB040 and NC versus BC1 and BC2.(B) Top differentially expressed genes (HUB040 and NC < BC1 and BC2) sorted on 2log difference (t-test, FDR corrected). mRNA expression of (C) DKK1, (D) CDH1, (E) FAM3D, and (F) SOSTDC1 in CRC TCGA dataset (BRAF-M, n = 57; BRAF-wt n = 467), and colon cancer dataset Marisa et al. (BRAF-M, n = 51; BRAF-wt n = 461).(G) Immunoblot analysis of FAM3D in HUB040, NC, BC1 and BC2. Unpaired t-test ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. CRC, colorectal cancer; TCGA, The Cancer Genome Atlas. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40687798), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human FAM3D Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human FAM3D

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FAM3D

FAM3D, also known as EF-7, belongs to the novel cytokine-like family whose members are secreted four-helix bundle proteins. Human FAM3D is abundantly expressed in placenta as a 224 amino acid (aa) residue precursor with a 25 aa signal sequence. The aa sequence of mature human FAM3D is 74% identical to that of mouse FAM3D.

Long Name

Family With Sequence Similarity 3, Member D

Alternate Names

EF7, OIT1

Entrez Gene IDs

131177 (Human); 18300 (Mouse)

Gene Symbol

FAM3D

UniProt

Q96BQ1

Additional FAM3D Products

Product Documents for Human FAM3D Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FAM3D Antibody

For research use only

Related Research Areas

Diabetes

Citations for Human FAM3D Antibody

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