Human FCRL4/FcRH4 Antibody

R&D Systems | Catalog # AF2426

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FCRL4/FcRH4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FCRL4/FcRH4
Gln16-Asp385
Accession # Q96PJ5

Specificity

Detects human FCRL4/FcRH4 in direct ELISAs. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) IRTA2, rhIRTA3, rhIRTA4, and rhIRTA5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FCRL4/FcRH4 Antibody

Detection of FCRL4/FcRH4 in Human Tonsil.

FCRL4/FcRH4 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Goat Anti-Human FCRL4/FcRH4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2426) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human FCRL4/FcRH4 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil

Reviewed Applications

Read 2 reviews rated 4 using AF2426 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRL4/FcRH4

FcRH4, also known as IRTA1 (immunoglobulin superfamily receptor translocation associated 1), is a member of the Ig superfamily. It shares sequence homology with the classical Fc receptors. FcRH4 is preferentially expressed in B cells and contains three potential ITIM motifs in its extracellular domain. The gene for FcRH4 is localized to the human chromosome Iq 21‑23 region, a hotspot for translocation events.

Long Name

Fc Receptor-like protein 4

Alternate Names

CD307d, FcRH4, IGFP2, IRTA1

Entrez Gene IDs

83417 (Human)

Gene Symbol

FCRL4

UniProt

Q96PJ5

Additional FCRL4/FcRH4 Products

Product Documents for Human FCRL4/FcRH4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FCRL4/FcRH4 Antibody

For research use only

Citations for Human FCRL4/FcRH4 Antibody

Customer Reviews for Human FCRL4/FcRH4 Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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1 Stars
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Customer Images

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  • Human FCRL4/FcRH4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lymphoma tissue
    Species: Human
    Verified Customer | Posted 12/13/2021
    Leica BOND autostainer retrieval: boiling in ER2 primary x50 30min detected with Simple stain MAX-PO (G)
    Human FCRL4/FcRH4 Antibody AF2426
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: See PMID 21908428
    Species: Human
    Verified Customer | Posted 01/06/2015

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Protocols

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