Human Fetuin B Antibody (212633) [Biotin]

R&D Systems | Catalog # BAM17251

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Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 212633
View all Fetuin B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fetuin B
Met19-Pro382
Accession # NP_055190

Specificity

Detects human Fetuin B in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Fetuin A, rhCystatin A, B, C, rhMMP-2, 9, rhBMP-2, 4, 6, rhTGF-beta 1, or rhTGF-beta 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fetuin B Antibody (212633) [Biotin]

Human Fetuin B ELISA Standard Curve

Human Fetuin B ELISA Standard Curve

Recombinant Human Fetuin B (Catalog # 1725-PI) was serially diluted and captured by Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Fetuin B Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Fetuin B Antibody (212633) [Biotin]

Human Fetuin B Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Fetuin B Antibody (Catalog # MAB17251)
  • Standard: Recombinant Human Fetuin B Protein, CF (Catalog # 1725-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fetuin B

Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1‑3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection (7). Fetuin B is a newer member whose function is not fully characterized (1, 2). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).

References

  1. Oliver, E. et al. (1999) Genomics. 57:352.
  2. Oliver, E. et al. (2000) Biochem. J. 350:589.
  3. Kellemann, J. et al. 1989, J. Biol. Chem. 264:14121.
  4. Denecke, B. et al. (2003) Biochem. J. 376:135.
  5. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
  6. Dziegielewska, K. M. et al. (1996) Histochem. Cell Biol. 106:319.
  7. Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.

Alternate Names

FETUB

Entrez Gene IDs

26998 (Human)

Gene Symbol

FETUB

UniProt

NP_055190

Additional Fetuin B Products

Product Documents for Human Fetuin B Antibody (212633) [Biotin]

Certificate of Analysis

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Product Specific Notices for Human Fetuin B Antibody (212633) [Biotin]

For research use only

Related Research Areas

Cysteine Proteases and Regulators

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