Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 19805
View all FGF-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-4
Ala31-Leu206
Accession # P08620

Specificity

Detects human FGF-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with bovine FGF acidic, recombinant human (rh) FGF acidic, bovine FGF basic, rhFGF basic, or rh beta -ECGF is observed. The antibody

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human FGF-4 Antibody (19805) [Alexa Fluor® 594]

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months, 2 to 8 °C under sterile conditions after opening.

    • Background: FGF-4

    FGF-4, the product of a developmentally regulated gene (hst-1), is a member of the FGF family that is efficiently secreted. The gene for FGF-4 (also known as FGFK or K-FGF for Kaposi sarcoma-associated FGF) was initially discovered as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors (including stomach and colon cancers, hepatocellular carcinomas, and Kaposi’s sarcomas). FGF-4 does not seem to be expressed in normal adult tissues. However, expression of the gene is spatially and temporally regulated during embryonic development. The murine homologue of human FGF-4 has been cloned and shown to be 82% homologous to the human protein at the amino acid sequence level. Human FGF-4 has been shown to exhibit cross species activity.

    In vitro, FGF-4 is mitogenic for fibroblasts and endothelial cells. FGF-4 has been shown to be a potent angiogenesis promoter in vivo. FGF-4 has potent transforming potential apparently through an autocrine mechanism of action. FGF-4 plays a key role in limb development and has been identified as the molecular mediator of the activities of the apical ectodermal ridge that is required for directing the outgrowth and patterning of vertebrate limbs.

    Long Name

    Fibroblast Growth Factor 4

    Alternate Names

    FGF4, HBGF-4, HST-1, KFGF, KS3

    Entrez Gene IDs

    2249 (Human); 14175 (Mouse); 116499 (Rat)

    Gene Symbol

    FGF4

    UniProt

    P08620

    Additional FGF-4 Products

    Product Documents for Human FGF-4 Antibody (19805) [Alexa Fluor® 594]

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Human FGF-4 Antibody (19805) [Alexa Fluor® 594]

    This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

    Recommended applications are based on validated applications from the unconjugated base product MAB235.

    For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.


    This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

    For research use only

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