Human FGFR2 (IIIb) Antibody (98707)

FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.

Recombinant Human FGF R2a (IIIb) Fc Chimera (Catalog # 663-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 (IIIb) Monoclonal Antibody (Catalog # MAB665). The ND₅₀ is typically 2-8 µg/mL in the presence of heparin (10 µg/mL).

Detection of FGFR2 (IIIb) by Western Blot

Ang2 reduces p-FGFR, p-Erk1/2, p-STAT3 and p-AKT in HEK293T cells. A, B HEK293T cells were incubated in starvation medium only (None), with Ang2 (3 nM), with FGF1 (3 nM), or with Ang2 (3 nM) + FGF1 (3 nM) for the indicated times (min). Cell lysates were immunoblotted with the indicated antibodies. Representative results from 3 experiments. When FGF1 + Ang2 were added to the cells, the incubation time of FGF1 was 5 min and the incubation time of Ang2 is either 1, 2 or 5 minutes (displayed as 5/1, 5/2, 5/5). C HEK293T cells were incubated in starvation medium with FGF2 (3 nM) alone (2 or 5 min), or with Ang2 (3 nM), 5 min. The results show Ang2 + FGF2 for 5 min reduce FGF2-induced Erk1/2 activity (5 min). D Left panel: HEK293T cells were incubated in starvation medium only (None), FGF1 only (5 nM; 5 min), or with Ang2 (5 nM; 5 min) without or with AMG386 (AMG, 5 ng/ml; 1, 2, or 5 min). Right panel: HEK293T cells were incubated with medium only (None), FGF1 only (5 nM; 5 min), or FGF1 (5 nM; 5 min) + Ang2 (5 nM; 5 min), with or without AMG386 (5 ng/ml; 1, 2, or 5 min). Relative band intensity (p-Erk/total Erk) is shown in the bar graph. Representative of 3 experiments Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1007/s10456-025-09988-2), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FGFR2 (IIIb) by Western Blot

Ang2 reduces p-FGFR, p-Erk1/2, p-STAT3 and p-AKT in HEK293T cells. A, B HEK293T cells were incubated in starvation medium only (None), with Ang2 (3 nM), with FGF1 (3 nM), or with Ang2 (3 nM) + FGF1 (3 nM) for the indicated times (min). Cell lysates were immunoblotted with the indicated antibodies. Representative results from 3 experiments. When FGF1 + Ang2 were added to the cells, the incubation time of FGF1 was 5 min and the incubation time of Ang2 is either 1, 2 or 5 minutes (displayed as 5/1, 5/2, 5/5). C HEK293T cells were incubated in starvation medium with FGF2 (3 nM) alone (2 or 5 min), or with Ang2 (3 nM), 5 min. The results show Ang2 + FGF2 for 5 min reduce FGF2-induced Erk1/2 activity (5 min). D Left panel: HEK293T cells were incubated in starvation medium only (None), FGF1 only (5 nM; 5 min), or with Ang2 (5 nM; 5 min) without or with AMG386 (AMG, 5 ng/ml; 1, 2, or 5 min). Right panel: HEK293T cells were incubated with medium only (None), FGF1 only (5 nM; 5 min), or FGF1 (5 nM; 5 min) + Ang2 (5 nM; 5 min), with or without AMG386 (5 ng/ml; 1, 2, or 5 min). Relative band intensity (p-Erk/total Erk) is shown in the bar graph. Representative of 3 experiments Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1007/s10456-025-09988-2), licensed under a CC-BY license. Not internally tested by R&D Systems.