Human Flt-3 Ligand/FLT3L Antibody (40406)

Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody.

Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK or 308-FKN) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (5 ng/mL) is neutralized (green line) by increasing concentrations of Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB308). The ND₅₀ is typically 0.02-0.06 µg/mL.

Detection of Flt-3 Ligand/FLT3L by Flow Cytometry

Expansion of human DC populations by Alb-Flt3L in humanized mice.NSG mice were irradiated and engrafted with human CD34+ cells according to Taconic’s established protocols. Successful chimerism was confirmed by flow cytometry. Three mice from each donor were generated, allowing for a donor-match control to be in each group (n = 3). Mice were administered Flt3L, Alb-Flt3L (equimolar amounts), or vehicle control every 5 days for a total of 3 doses. Mice were then euthanized, and spleens and bone marrow were harvested and used for immune cell analysis. Representative flow gating and percentages of total DCs gated as CD11c+HLA-DR+ (A and D), cDC1s gated as CD11c+HLA-DR+CD141+ (B and F), cDC2s gated as CD11c+HLA-DR+CD1c+ (B and G), and pDCs gated as CD11c+HLA-DRloCD123+CD303+ (C and E). Representative SPADE analysis of flow cytometry data collected from (H) spleen or (I) bone marrow of mice treated with vehicle, Flt3L, or Alb-Flt3L (equimolar amount). Donor-matched controls are shown. Vehicle was used to set baseline cell populations in each cluster. scRNA-seq data collected from donor-matched spleens of mice treated with vehicle or Alb-Flt3L. UMAP projection of scRNA-seq data from (J) vehicle-treated (naive) or (K) Alb-Flt3L–treated mice. Mono, monocytes. (L) Heatmap showing gene cluster differences in mice treated with Alb-Flt3L compared with control. *P < 0.05; **P < 0.01; ***P < 0.001 by 2-tailed Student’s t test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37917174), licensed under a CC-BY license. Not internally tested by R&D Systems.