Human FOLR1 Antibody (548908)

R&D Systems | Catalog # MAB5646

R&D Systems
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 548908
View all FOLR1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
Arg25-Met233
Accession # P15328

Specificity

Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FOLR1 Antibody (548908)

Detection of Human FOLR1 antibody by Western Blot.

Detection of Human FOLR1 by Western Blot.

Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only.
Detection of FOLR1 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of FOLR1 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins.
FOLR1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

FOLR1 in MCF-7 Human Cell Line.

FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
FOLR1 Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line.

FOLR1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). No staining in the FOLR1 knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Detection of Human FOLR1 by Immunocytochemistry/Immunofluorescence

Detection of Human FOLR1 by Immunocytochemistry/Immunofluorescence

(A) Immunofluorescent (left) and flow cytometry (right) detection of the EpCAM expression in MCF7 cells and the FR alpha expression in A2780 cells. EpCAM and FR alpha stained with Alexa Fluor® 488 are green at an excitation of 488 nm, and the nuclei stained with DAPI are blue at an excitation of 405 nm. As a negative control, we used EpCAM to stain Jurkat cells, which are EpCAM negatively expressed, and we used FR alpha to stain A549 cells, which are FR alpha negatively expressed. Histograms of flow cytometric analysis: MCF7 cells (top) were stained with anti-EpCAM antibodies (red) and A2780 cells (bottom) were stained with anti-FR alpha antibodies (red), the negative control was autofluorescent (orange), and the isotype was mouse IgG plus secondary antibody (blue). (B) Schematic of our CTCs enrichment strategy. Anti-EpCAM-MNs and Anti-FR alpha -MNs was added to the whole blood and after incubation, magnetic separation and fluorescence identification, the cells of DAPI+/CK+/CD45− were defined as CTC. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29352248), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FOLR1 in HeLa cells by Flow Cytometry

HeLa cells were stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Human FOLR1 ELISA Standard Curve

Human FOLR1 ELISA Standard Curve

Recombinant Human FOLR1 (Catalog # 5646-FR) was serially diluted and captured by Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FOLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5646) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human FOLR1 Antibody (548908)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MCF‑7 human breast cancer cell line; HeLa human cervical epithelial carcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Knockout Validated

FOLR1 is specifically detected in MCF-7 human breast cancer parental cell line but is not detectable in FOLR1 knockout MCF-7 cell line.

Western Blot

2 µg/mL
Sample: Human cortex tissue and HeLa human cervical epithelial carcinoma cell line under non-reducing conditions only

Human FOLR1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human FOLR1 Biotinylated Antibody (Catalog # BAF5646)
  • Standard: Recombinant Human FOLR1 Protein, CF (Catalog # 5646-FR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 8 reviews rated 3.9 using MAB5646 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).

References

  1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
  4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
  5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
  6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
  9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
  11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Long Name

Folate Receptor 1

Alternate Names

FBP, Folbp1, FR-alpha, MOv18

Entrez Gene IDs

2348 (Human); 14275 (Mouse); 171049 (Rat)

Gene Symbol

FOLR1

UniProt

P15328 (Human)

Additional FOLR1 Products

Product Documents for Human FOLR1 Antibody (548908)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FOLR1 Antibody (548908)

For research use only

Related Research Areas

Cancer Biomarkers
Neuronal Markers

Citations for Human FOLR1 Antibody (548908)

Customer Reviews for Human FOLR1 Antibody (548908) (8)

3.9 out of 5
8 Customer Ratings
5 Stars
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4 Stars
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2 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • Human FOLR1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell line
    Species: Human
    Verified Customer | Posted 08/26/2021
    Human FOLR1 Antibody (548908) MAB5646
  • Human FOLR1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: IGROV1 cell line
    Species: Human
    Verified Customer | Posted 04/10/2019
    Human FOLR1 Antibody (548908) MAB5646
  • Human FOLR1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: IGROV cell line and IGROV1 cell line
    Species: Human
    Verified Customer | Posted 04/07/2019
    Human FOLR1 Antibody (548908) MAB5646
  • Human FOLR1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell line
    Species: Human
    Verified Customer | Posted 10/04/2018
    After very long exposure gave a band of an expected size. Very weak signal even with ECL Plus.
    Human FOLR1 Antibody (548908) MAB5646
  • Human FOLR1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: SK-OV-3 human ovarian adenocarcinoma cell line, igrov-1, NCI-H292, HT-29 human colon adenocarcinoma cell line and CAOV3
    Species: Human
    Verified Customer | Posted 05/08/2018
    Human FOLR1 Antibody (548908) MAB5646
  • Human FOLR1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: human cancer cell line
    Species: Human
    Verified Customer | Posted 04/03/2018
    Human FOLR1 Antibody (548908) MAB5646
  • Human FOLR1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human cancer cell line
    Species: Human
    Verified Customer | Posted 03/21/2018
    Human FOLR1 Antibody (548908) MAB5646
  • Human FOLR1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Ovarian cancer tissue
    Species: Human
    Verified Customer | Posted 02/23/2016
    Human FOLR1 Antibody (548908) MAB5646
    Bio-Techne Response
    R&D Systems Technical Service will investigate.

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