Human FoxC1 Antibody (625905)

R&D Systems | Catalog # MAB6329

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 625905
View all FoxC1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxC1
Gln208-Gln322
Accession # Q12948

Specificity

Detects human FoxC1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FoxC1 Antibody (625905)

Detection of Human FoxC1 antibody by Western Blot.

Detection of Human FoxC1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, NCI-H460 human large cell lung carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human FoxC1 Monoclonal Antibody (Catalog # MAB6329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human FoxC1 Antibody (625905)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, NCI‑H460 human large cell lung carcinoma cell line, and HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxC1

Human FoxC1 (forkhead box C1), also called FKHL7 (forkhead-like 7) or FREAC3 (forkhead-related activator 3) is a 553 amino acid (aa) intracellular phosphoprotein that belongs to a large family of nuclear transcription factors that share a common forkhead/winged helix DNA binding domain. FoxC1 is essential for formation of mesodermal tissues; mutations underlie Axenfeld-Rieger malformations of the eye. The human FoxC1 sequence includes a DNA binding domain at aa 77‑168 and an inhibitory domain between aa 268‑245. Phosphorylation within this inhibitory region causes a protein gel mobility shift. Human FoxC1 shares 92% and 91% aa identity with mouse and rat FoxC1, respectively.

Long Name

Forkhead Box C1

Alternate Names

ARA, FKHL7, FREAC3, IGDA, IHG1, IRID1, RIEG3

Entrez Gene IDs

2296 (Human); 17300 (Mouse)

Gene Symbol

FOXC1

UniProt

Q12948 (Human)

Additional FoxC1 Products

Product Documents for Human FoxC1 Antibody (625905)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FoxC1 Antibody (625905)

For research use only

Citations for Human FoxC1 Antibody (625905)

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