Human FXR/NR1H4 Antibody (A9033A)

R&D Systems | Catalog # PP-A9033A-00

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Direct ELISA, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # A9033A
View all FXR/NR1H4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human FXR/NR1H4
aa 2-126

Specificity

This antibody specifically recognizes human FXR and cross-reacts with rat and mouse FXR. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human FXR/NR1H4 Antibody (A9033A)

Detection of FXR/NR1H4 by Western Blot

Detection of FXR/NR1H4 by Western Blot

Generation of CRISPR knockout FXR mice. (A) Single cell embryos were injected with guide RNA that targets exon 5 of Fxr. The embryos were implanted into pseudo-pregnant females to generate founders. The sequencing results show that the mutation was a 47 base-pair deletion at exon 5. (B) Hepatocytes were isolated from control and Fxrex5 delta livers using the classic collagenase-perfusion method. Western blot was performed on whole cell lysates from control and Fxrex5 delta hepatocytes. VINCULIN was used as a loading control. (C) RNA-seq was performed on control and Fxr ex5 delta hepatocytes. Heatmap of 789 differentially expressed genes between control and Fxrex5 delta hepatocytes (p value and FDR value cut-off of 0.05). (D) Overlap of dysregulated genes from Fxrex5 delta hepatocyte RNAseq with Fxrex5 delta liver RNAseq obtained from Annak et al. (2011) [22]. Pathways analysis of all upregulated and downregulated genes was conducted using the KEGG and REACTOME databases. The cut-off for pathways analysis was chosen as p-value < 0.05, FDR < 0.1. The dotted line represents a FDR value of 0.05. Any value on the right of the dotted line signifies pathways that pass the FDR threshold of 0.05. (E) Hepatic and serum bile acid analysis at 4-week, 4-month and 12-month time-points in control and Fxrex5 delta animals (n = 5). (F) Expression analysis of selected downregulated and upregulated genes upon FXR deletion. The normalized FPKM values from RNAseq analysis were averaged for control and Fxrex5 delta hepatocytes and fold-changes were calculated (n = 3). T-test was performed to determine statistical significance relative to controls: * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36551676), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human FXR/NR1H4 Antibody (A9033A)

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.2 μg/mL with the appropriate secondary reagents to detect human FXR.

Immunohistochemistry

This antibody can be used at 20-40 μg/mL with the appropriate secondary reagents to detect human FXR.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human FXR.

Reviewed Applications

Read 3 reviews rated 5 using PP-A9033A-00 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN<sub>3</sub>

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: FXR/NR1H4

Farnesoid X-activated Receptor (FXR, HRR-1, BAR, RIP14; NR1H4) is a member of the Orphan Nuclear Receptor family. FXR is expressed in the liver, intestinal villi, renal tubes and adrenal cortex. FXR is a global regulator of bile acid metabolism. Two genes, cholesterol 7a-hydroxylase (CYP7A1) and IBABP (Ileal Bile Acid Binding Protein), which are implicated in bile acid biosynthesis and recycling, respectively, are target genes of FXR. FXR is shown to be transcriptionally activated by farnesol metabolites such as farnesol itself and juvenile hormone III. FXR binds to DNA only as a heterodimer with Retinoid X Receptor (RXR).

Long Name

Farnesoid X-activated receptor

Alternate Names

BAR, HRR-1, NR1H4, RIP14

Entrez Gene IDs

9971 (Human); 20186 (Mouse); 60351 (Rat)

Gene Symbol

NR1H4

Additional FXR/NR1H4 Products

Product Documents for Human FXR/NR1H4 Antibody (A9033A)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FXR/NR1H4 Antibody (A9033A)

For research use only

Citations for Human FXR/NR1H4 Antibody (A9033A)

Customer Reviews for Human FXR/NR1H4 Antibody (A9033A) (3)

5 out of 5
3 Customer Ratings
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4 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human FXR/NR1H4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Leydig cells
    Species: Human
    Verified Customer | Posted 08/31/2021
    Human FXR/NR1H4 Antibody (A9033A) PP-A9033A-00
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23056173
    Species: Human
    Verified Customer | Posted 02/25/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23530060
    Species: Human
    Verified Customer | Posted 02/25/2015

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