Human GAD2/GAD65 Antibody

R&D Systems | Catalog # AF2247

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunocytochemistry, Bioassay, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GAD2/GAD65 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GAD2/GAD65
Ala1-Ala150
Accession # Q05329

Specificity

Detects human GAD2/GAD65 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human GAD1/GAD67 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GAD2/GAD65 Antibody

GAD2/GAD65 antibody in Human Dorsal Root Ganglia by Immunohistochemistry (IHC-P).

GAD2/GAD65 in Human Dorsal Root Ganglia.

GAD2/GAD65 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using Goat Anti-Human GAD2/GAD65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2247) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human GAD2/GAD65 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human dorsal root ganglia

Western Blot

0.1 µg/mL
Sample: Recombinant Human GAD2/GAD65

Reviewed Applications

Read 1 review rated 5 using AF2247 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GAD2/GAD65

Glutamic acid decarboxylases (GAD), which catalyze the formation of gamma-aminobutyric acid from L-glutamic acid, are detectable in different isoforms encoded by distinct genes. GAD2, also known as GAD65, is expressed in the brain and pancreatic islets. GAD2 has been implicated as an autoantigen in the autoimmune disease Stiff Man Syndrome and in insulin-dependent diabetes mellitus.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Entrez Gene IDs

2572 (Human)

Gene Symbol

GAD2

UniProt

Q05329

Additional GAD2/GAD65 Products

Product Documents for Human GAD2/GAD65 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GAD2/GAD65 Antibody

For research use only

Citations for Human GAD2/GAD65 Antibody

Customer Reviews for Human GAD2/GAD65 Antibody (1)

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  • Human GAD2/GAD65 Antibody
    Name: Lydia Taverne
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Adult brain
    Species: Human
    Verified Customer | Posted 12/16/2019
    1:50 GAD65 staining on normal human brain tissue shows distinct membranous & cytoplasmic positivity in the granular layer.
    Concentration of 1:50 (4 ug/mL) GAD65 antibody on normal human brain tissue, with 488-labeled secondary antibody (green fluor). Antigen retrieval was with citrate pH 6 buffer
    Human GAD2/GAD65 Antibody AF2247

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Protocols

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