Human GAK Antibody (706306)

R&D Systems | Catalog # MAB6918

R&D Systems
Discontinued Product
MAB6918 has been discontinued. View all GAK products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 706306
View all GAK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GAK
Phe258-Asp443
Accession # O14976

Specificity

Detects human GAK in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GAK Antibody (706306)

Detection of Human GAK antibody by Western Blot.

Detection of Human GAK by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and BJAB human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GAK Monoclonal Antibody (Catalog # MAB6918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAK at approximately 144 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GAK Antibody (706306)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and BJAB human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GAK

Cyclin G-associated kinase (GAK), also known as Auxilin 2, is an approximately 140 kDa widely expressed protein that associates with CDK5. GAK promotes microtubule growth during mitotic spindle assembly and is required for centrosome maturation, chromosome congression, and mitotic progression. It also regulates the assembly and intracellular trafficking of clathrin-coated vesicles. GAK contains a Ser/Thr protein kinase domain (aa 40-314), a phosphatase tensin/auxilin domain (aa 399-566), a PTEN C2 domain (aa 570-708), and a J domain (aa 1247-1311). Within aa 258-443, human GAK shares 88% aa sequence identity with mouse and rat GAK.

Long Name

Cyclin G-associated Kinase

Alternate Names

Auxillin 2

Entrez Gene IDs

2580 (Human); 231580 (Mouse); 81659 (Rat)

Gene Symbol

GAK

UniProt

O14976

Additional GAK Products

Product Documents for Human GAK Antibody (706306)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GAK Antibody (706306)

For research use only

Citations for Human GAK Antibody (706306)

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