The neuropeptide galanin elicits a range of biological effects by interaction with specific G protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.
Human Galanin R1/GALR1 Antibody (613207)
R&D Systems | Catalog # MAB10353
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 613207
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Galanin R1/GALR1
Accession # P47211
Accession # P47211
Specificity
Detects human Galanin R1/GALR1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Galanin R1/GALR1 Antibody (613207)
Detection of Galanin R1/GALR1 in HEK293 Human Cell Line Transfected with Human Galanin R1/GALR1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human Galanin R1/GALR1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Galanin R1/GALR1 Monoclonal Antibody (Catalog # MAB10353) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.Applications for Human Galanin R1/GALR1 Antibody (613207)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Galanin R1/GALR1 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human Galanin R1/GALR1 and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Galanin R1/GALR1
Long Name
Galanin Receptor Type 1
Alternate Names
GAL1, GalaninR1, GALNR1, GALR1
Gene Symbol
GALR1
UniProt
Additional Galanin R1/GALR1 Products
Product Documents for Human Galanin R1/GALR1 Antibody (613207)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Galanin R1/GALR1 Antibody (613207)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
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- Flow Cytometry Troubleshooting Guide
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- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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