Human GATA-4 Antibody (532020)

R&D Systems | Catalog # MAB2606

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Callithrix jacchus (Common Marmoset), Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 532020
View all GATA-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694

Specificity

Detects human GATA-4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GATA-4 Antibody (532020)

Detection of Human GATA‑4 antibody by Western Blot.

Detection of Human GATA‑4 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GATA-4 Monoclonal Antibody (Catalog # MAB2606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GATA-4 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GATA-4 Antibody (532020)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and human liver tissue

Reviewed Applications

Read 2 reviews rated 5 using MAB2606 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-4

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. GATA-4 contains 2 zinc finger-binding domains and migrates at 56 kDa in SDS-PAGE gels. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Alternate Names

GATA4

Entrez Gene IDs

2626 (Human)

Gene Symbol

GATA4

UniProt

P43694

Additional GATA-4 Products

Product Documents for Human GATA-4 Antibody (532020)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA-4 Antibody (532020)

For research use only

Citations for Human GATA-4 Antibody (532020)

Customer Reviews for Human GATA-4 Antibody (532020) (2)

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2 Customer Ratings
5 Stars
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4 Stars
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  • Human GATA-4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult pancreas
    Species: Mouse
    Verified Customer | Posted 12/10/2018
    Paraffin-embedded sections Antigen retrieval: Citrate pH=6 Mouse pancreas
    Human GATA-4 Antibody (532020) MAB2606
  • Human GATA-4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 03/31/2018

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