Human GLI-3 Antibody (2352B)

R&D Systems | Catalog # MAB3690

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2352B
View all GLI-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071

Specificity

Detects human GLI-3 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human GLI-3 Antibody (2352B)

Detection of GLI-3 in Human SW480 cell line.

GLI‑3 was detected in immersion fixed  SW480 fibroblast carcinoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Rabbit Anti-Human GLI‑3 Monoclonal Antibody (Catalog # MAB3690) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GLI-3 Antibody (2352B)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed SW480 human fibroblast carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GLI-3

GLI3 is a zinc finger protein.  The GLI family of proteins are transcription factors and are mediators of Sonic hedgehog signaling.  GLI3 is known to be a transcriptional repressor but also may have a positive transcription function. Mutations in the GLI3 gene are associated with many polydactyly diseases.

References

Rupert JM., Vogelstein B., Arheden K., Kinzler KW., "GLI3 Encodes a 190-kilodalton Protein with Multiple Regions of GLI Similarity". Molecular and Cellular Biology. 1990 Oct; 10(10):  5408-15. Taipale J., Beachy PA., "The Hedgehog and Wnt Signaling Pathways in Cancer".  Nature.  2001 May;411(6835):  349-54. Rash BG., Grove EA., "Patterning the Dorsal Telencephalon: a Role for Sonic Hedgehog ?".  The Journal of Neuroscience.  2007 Oct; 27(43):  11595-603.

Long Name

GLI-Kruppel family member GLI3

Alternate Names

ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt

Entrez Gene IDs

2737 (Human); 14634 (Mouse); 140588 (Rat)

Gene Symbol

GLI3

UniProt

P10071

Additional GLI-3 Products

Product Documents for Human GLI-3 Antibody (2352B)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GLI-3 Antibody (2352B)

For research use only

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail