Human Glut1 Antibody (202921)

Detection of Human Glut1 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Glut1 by Simple Western^TM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Detection of Glut1 by Western Blot

Extracellular matrix stiffness modulates the expression of monocarboxylate transporters in mammary stromal cells. RMF 621 cells (2.5 × 105 per plate) were incubated on plates displaying increasing stiffness and coated with collagen I, as described above, in media +10% FCS. Afterward, 72 cells were lysed and Western blots of MCT4 (A) and Glut1 (B) were performed as described in Section 4. Densitometric analyses of three different experiments were performed from three independent experiments, as shown in (C). The data are presented as the means ± standard error (SEM). (*) and (**) indicate statistically significant differences, with p ≤ 0.05 and p ≤ 0.01, respectively, using the Friedman test followed by Dunn’s posthoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34357326), licensed under a CC-BY license. Not internally tested by R&D Systems.