Human Glypican 4 Antibody (961609)

R&D Systems | Catalog # MAB9195

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 961609
View all Glypican 4 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Glypican 4
Met1-Ser529
Accession # O75487

Specificity

Detects human Glypican 4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Glypican 4 Antibody (961609)

Detection of Glypican 4 antibody in Human TF-1 Cell Line antibody by Flow Cytometry.

Detection of Glypican 4 in Human TF-1 Cell Line by Flow Cytometry.

TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB9195, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of Glypican 4 antibody in Human BG01V Cells antibody by Flow Cytometry.

Detection of Glypican 4 in Human BG01V Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB9195, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Glypican 4 Antibody (961609)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB91951).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/106 cells
Sample: TF-1 Human Erythroleukemic Cell Line, and BG01V Human Embryonic Stem Cell

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glypican 4

Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).

References

  1. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
  2. Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
  3. Ussar, S. et al. (2012) Diabetes 61:2289.
  4. Fico, A. et al. (2014) J. Neurosci. 34:8318.
  5. Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
  6. Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
  7. Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
  8. Strate, I. et al. (2015) Development 142:1767.

Alternate Names

GPC4, K-glypican

Entrez Gene IDs

2239 (Human); 14735 (Mouse); 317322 (Rat)

Gene Symbol

GPC4

UniProt

O75487

Additional Glypican 4 Products

Product Documents for Human Glypican 4 Antibody (961609)

Certificate of Analysis

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Product Specific Notices for Human Glypican 4 Antibody (961609)

For research use only

Citations for Human Glypican 4 Antibody (961609)

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Protocols

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