Human GM130/GOLGA2 Antibody

R&D Systems | Catalog # AF8199

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GM130/GOLGA2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GM130/GOLGA2
Met528-Gln606
Accession # Q08379

Specificity

Detects human GM130/GOLGA2 in direct ELISAs and Western blots.   

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human GM130/GOLGA2 Antibody

Detection of Human GM130/GOLGA2 antibody by Western Blot.

Detection of Human GM130/GOLGA2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and ZR-75 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GM130/GOLGA2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

GM130/GOLGA2 in HeLa Human Cell Line.

GM130/GOLGA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human GM130/GOLGA2 antibody by Simple WesternTM.

Detection of Human GM130/GOLGA2 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and ZR-75 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for GM130/GOLGA2 at approximately 141 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse GM130/GOLGA2 by Immunocytochemistry/Immunofluorescence

Detection of Mouse GM130/GOLGA2 by Immunocytochemistry/Immunofluorescence

Kcnj13 orchestrates SM cell alignment and polarity. a Immunostaining for alpha SMA (red) and DAPI staining (blue) in dorsal views of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheas. b Quantification of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheal SM cell orientation. c Quantification of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheal SM cell nuclear aspect ratio (NAR). d Immunostaining for alpha SMA (red) and DAPI staining (blue) of transverse sections of E14.5 WT (n = 10) and Kcnj13T38C/T38C (n = 10) tracheas. e Quantification of WT (n = 10) and Kcnj13T38C/T38C (n = 10) tracheal SM cell layers. f Immunostaining for alpha SMA (red) and GM130 (green) and DAPI staining (blue) in dorsal views of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheas. g Quantification of WT (n = 8) and Kcnj13T38C/T38C (n = 8) Golgi apparatus (green) position relative to the nucleus (blue). h Timeline for tamoxifen administration. i Immunostaining for KCNJ13 (red), alpha SMA (green), and DAPI staining (blue) of transverse sections of E14.5 control (n = 6) and Myh11-CreERT2;Kcnj13flox/flox (n = 6) tracheas. j Representative images of ventral views of E14.5 control (n = 9) and Myh11-CreERT2;Kcnj13flox/flox (n = 9) tracheas. Double-sided arrows indicate tracheal tube length. k Quantification of E14.5 control (n = 9) and Myh11-CreERT2;Kcnj13flox/flox (n = 9) tracheal tube length. l Immunostaining for alpha SMA (red) and DAPI staining (blue) in dorsal views of E14.5 control (n = 9) and Myh11-CreERT2;Kcnj13flox/flox (n = 9) tracheas. m Quantification of E14.5 control (n = 9) and Myh11-CreERT2;Kcnj13flox/flox (n = 9) tracheal SM cell orientation. n Immunostaining for SOX9 (green) in ventral views of E15.5 control (n = 6) and Myh11-CreERT2;Kcnj13flox/flox (n = 6) tracheas. Arrows point to tracheal mesenchymal condensations. Scale bars: 1000 μm (j), 100 μm (n), 50 μm (d), 20 μm (a, f, i, l). *P < 0.05; **P < 0.01; unpaired Student’s t-test, mean ± s.d Image collected and cropped by CiteAb from the fol

Applications for Human GM130/GOLGA2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Simple Western

50 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and ZR‑75 human breast cancer cell line

Western Blot

0.25 µg/mL
Sample: MCF‑7 human breast cancer cell line and ZR‑75 human breast cancer cell line

Reviewed Applications

Read 3 reviews rated 4 using AF8199 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM130/GOLGA2

GM130, also known as Golgin subfamily A member 2 and GOLGA2, is a 130 kDa member of the Golgin protein family.  GM130/GOLGA2 is a component of the Golgi membrane involved in maintaining cis-Golgi structure.  GM130/GOLGA2 protein is thought to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been predicted. Human GM130/GOLGA2 protein is 1002 amino acids (aa) in length, and over aa 528‑606, shares 90% sequence identity with its mouse and rat orthologs.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Entrez Gene IDs

2801 (Human); 99412 (Mouse)

Gene Symbol

GOLGA2

UniProt

Q08379

Additional GM130/GOLGA2 Products

Product Documents for Human GM130/GOLGA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GM130/GOLGA2 Antibody

For research use only

Related Research Areas

Inflammatory Mediators

Citations for Human GM130/GOLGA2 Antibody

Customer Reviews for Human GM130/GOLGA2 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human GM130/GOLGA2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 10/06/2021
    Human GM130/GOLGA2 Antibody AF8199
  • Human GM130/GOLGA2 Antibody
    Name: Karina Kinghorn
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 07/16/2021
    Human GM130/GOLGA2 Antibody AF8199
  • Human GM130/GOLGA2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human primary fibroblast
    Species: Human
    Verified Customer | Posted 11/08/2020
    GM130 in human primary fibroblasts (Golgi). ICC using recently reconstituted GM130. It precipitates becoming useless in 6 month at -20ºC. PFA 4% fixed cells. Primary ab at 5 ug/mL O/N 4ºC. Donkey anti-Sheep Alexafluor 488.
    Good antbody to label Golgi in multiple ICC because it is produced in Sheep. It comes lyophilized, and after reconstitution and saved at -20ºC it precipitates in 6 month, becoming useless. ICC conditions: PFA 4% 20 min fixed cells. Blocking 10% DS in PBST (0.1% Triton) 1h RT. Primary ab O/N incubation 4ºC 5 ug/mL in DS 10% in PBST (0.1% Triton). Secondary Donkey anti-Sheep Alexa fluor 488 1:500 1h RT.
    Human GM130/GOLGA2 Antibody AF8199

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Protocols

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