Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody (735301)

R&D Systems | Catalog # MAB6487

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 735301
View all Hematopoietic Prostaglandin D Synthase/HPGDS Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Hematopoietic Prostaglandin D Synthase/HPGDS
Pro2-Leu199
Accession # O60760

Specificity

Detects human Hematopoietic Prostaglandin D Synthase/HPGDS in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody (735301)

Detection of Human Hematopoietic Prostaglandin D Synthase/HPGDS antibody by Western Blot.

Detection of Human Hematopoietic Prostaglandin D Synthase/HPGDS by Western Blot.

Western blot shows lysates of NCI-H460 human large cell lung carcinoma cell line, human lung tissue, and human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Hematopoietic Prostaglandin D Synthase/HPGDS Monoclonal Antibody (Catalog # MAB6487) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hematopoietic Prostaglandin D Synthase/HPGDS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hematopoietic Prostaglandin D Synthase/HPGDS antibody in HEL 92 by Immunocytochemistry (ICC).1 by Immunocytochemistry (ICC).7 Human Cell Line by Immunocytochemistry (ICC).

Hematopoietic Prostaglandin D Synthase/HPGDS in HEL 92.1.7 Human Cell Line.

Hematopoietic Prostaglandin D Synthase/HPGDS was detected in immersion fixed HEL 92.1.7 human erythroleukemic cell line using Mouse Anti-Human Hematopoietic Prostaglandin D Synthase/HPGDS Monoclonal Antibody (Catalog # MAB6487) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody (735301)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEL 92.1.7 human erythroleukemic cell line

Western Blot

0.25 µg/mL
Sample: NCI‑H460 human large cell lung carcinoma cell line, human lung tissue, and human placenta tissue

Reviewed Applications

Read 1 review rated 3 using MAB6487 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hematopoietic Prostaglandin D Synthase/HPGDS

Prostaglandin D Synthase (PGDS) catalyzes the conversion of prostaglandin (PG) H2 to PGD2, which is a major prostanoid produced in a variety of tissues. Two types of PGDS have been isolated; the glutathione-dependent hematopoietic PGDS (HPGDS) and the glutathione-independent lipocalin‑type PGDS (1). HPGDS is a cytosolic enzyme that is expressed in mast cells and antigen presenting cells (2, 3). It is the only mammalian member of the class Sigma glutathione S‑transferase, showing a broad specificity towards standard transferase substrates (4). The PGD2 produced by HPGDS is involved in many physiological processes such as maintaining body temperature, promotion of sleep, inhibition of platelet aggregation and bronchoconstriction (5). It also functions in immune response and acts as a mediator in allergy and inflammation (6). HPGDS‑specific inhibitors may be therapeutically useful anti‑allergic and anti‑inflammatory drugs.

References

  1. Urade, Y. and Eguchi, N. (2002) Prostaglandins Other Lipid Mediat. 68:375.
  2. Urade, Y. et al. (1990) J. Biol. Chem. 265:371.
  3. Urade, Y. et al. (1989) J. Immunol. 143:2982.
  4. Jowsey, I. R. et al. (2001) Biochem. J. 359:507.
  5. Kanaoka, Y. and Urade, Y. (2003) Prostaglandins Leukot. Essent. Fatty Acids 69:163.
  6. Oguma, T. et al. (2008) Allergol. Int. 57:307.

Alternate Names

GSTS, PGDS, PTGDS2

Entrez Gene IDs

27306 (Human); 54486 (Mouse); 58962 (Rat)

Gene Symbol

HPGDS

UniProt

O60760

Additional Hematopoietic Prostaglandin D Synthase/HPGDS Products

Product Documents for Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody (735301)

Certificate of Analysis

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Product Specific Notices for Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody (735301)

For research use only

Citations for Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody (735301)

Customer Reviews for Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody (735301) (1)

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  • Human Hematopoietic Prostaglandin D Synthase/HPGDS Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/04/2018

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